Th1/Th2 Cytokine Kit

Catalog No. ABIN1379790

Product Details

Target: IL-2, IL-4, IL-5, IFN-Gamma, TNF

Reactivity: Mouse

Application: Immunoassay (IA), Flow Cytometry (FACS)

Brand: BD CBA™

Characteristics: The BD™ CBA Mouse Th1/Th2 Cyokine Kit can be used to measure Interleukin-2 (IL-2), Interleukin-4 (IL-4), Interleukin-5 (IL-5), Interferon-gamma (IFN-gamma), and Tumor Necrosis Factor (TNF) protein levels in a single sample. The kit performance has been optimized for analysis of physiologically relevant concentrations (pg/mL levels) of specific cytokine proteins in tissue culture supernatants and serum samples. The kit provides sufficient reagents for the quantitative analysis of 80 samples

Components: Reagents for capture and detection of Reagents for capture and detection of IL-2, IL-4, IL-5, IFN-gamma, and TNF, standards, wash buffer, assay diluent, cytometer setup reagents, wash buffer, assay diluent, serum enhancement buffer, cytometer setup reagents

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C

References

Cook, Stahl, Lowe, Chen, Morgan, Wilson, Varro, Chan, Graziano, Barney: "Simultaneous measurement of six cytokines in a single sample of human tears using microparticle-based flow cytometry: allergics vs. non-allergics." in: Journal of immunological methods, Vol. 254, Issue 1-2, pp. 109-18, (2001) (PubMed).

Dotti, Savoldo, Takahashi, Goltsova, Brown, Rill, Rooney, Brenner: "Adenovector-induced expression of human-CD40-ligand (hCD40L) by multiple myeloma cells. A model for immunotherapy." in: Experimental hematology, Vol. 29, Issue 8, pp. 952-61, (2001) (PubMed).

Lund-Johansen, Davis, Bishop, de Waal Malefyt: "Flow cytometric analysis of immunoprecipitates: high-throughput analysis of protein phosphorylation and protein-protein interactions." in: Cytometry, Vol. 39, Issue 4, pp. 250-9, (2000) (PubMed).

Chen, Lowe, Wilson, Crowther, Tzeggai, Bishop, Varro: "Simultaneous Quantification of Six Human Cytokines in a Single Sample Using Microparticle-based Flow Cytometric Technology." in: Clinical chemistry, Vol. 45, Issue 9, pp. 1693-1694, (1999) (PubMed).

Carson, Vignali: "Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay." in: Journal of immunological methods, Vol. 227, Issue 1-2, pp. 41-52, (1999) (PubMed).

Bishop, Davis: "A flow cytometric immunoassay for beta2-microglobulin in whole blood." in: Journal of immunological methods, Vol. 210, Issue 1, pp. 79-87, (1998) (PubMed).

Oliver, Kettman, Fulton: "Multiplexed analysis of human cytokines by use of the FlowMetrix system." in: Clinical chemistry, Vol. 44, Issue 9, pp. 2057-60, (1998) (PubMed).

Fulton, McDade, Smith, Kienker, Kettman: "Advanced multiplexed analysis with the FlowMetrix system." in: Clinical chemistry, Vol. 43, Issue 9, pp. 1749-56, (1997) (PubMed).

Target Details

Target: IL-2, IL-4, IL-5, IFN-Gamma, TNF

Sub Type: Cocktail