Inflammation Kit

Catalog No. ABIN1379793

Product Details

Target: IL-8, IL-1beta, IL-6, IL-10, TNF, IL-12p70

Reactivity: Human

Application: Immunoassay (IA), Flow Cytometry (FACS)

Brand: BD CBA™

Characteristics: The BD™ CBA Human Inflammatory Cytokines Kit can be used to quantitatively measure interleukin-8 (IL-8), interleukin-1beta (IL-1beta), interleukin-6 (IL-6), interleukin- 10 (IL-10), tumor necrosis factor (TNF), and interleukin-12p70 (IL-12p70) protein levels in a single sample. The kit performance has been optimized for analysis of specific proteins in tissue culture supernatants, EDTAtreated plasma, and serum samples using one of two protocols, depending on the sample source. The kit provides sufficient reagents for 80 tests.

Components: Reagents for capture and detection of IL-8, IL-1beta, IL-6, IL-10, TNF, and IL-12p70, wash buffer, assay diluent, serum enhancement buffer, cytometer setup reagents

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C

References

Cook, Stahl, Lowe, Chen, Morgan, Wilson, Varro, Chan, Graziano, Barney: "Simultaneous measurement of six cytokines in a single sample of human tears using microparticle-based flow cytometry: allergics vs. non-allergics." in: Journal of immunological methods, Vol. 254, Issue 1-2, pp. 109-18, (2001) (PubMed).

Dotti, Savoldo, Takahashi, Goltsova, Brown, Rill, Rooney, Brenner: "Adenovector-induced expression of human-CD40-ligand (hCD40L) by multiple myeloma cells. A model for immunotherapy." in: Experimental hematology, Vol. 29, Issue 8, pp. 952-61, (2001) (PubMed).

Lund-Johansen, Davis, Bishop, de Waal Malefyt: "Flow cytometric analysis of immunoprecipitates: high-throughput analysis of protein phosphorylation and protein-protein interactions." in: Cytometry, Vol. 39, Issue 4, pp. 250-9, (2000) (PubMed).

Carson, Vignali: "Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay." in: Journal of immunological methods, Vol. 227, Issue 1-2, pp. 41-52, (1999) (PubMed).

Chen, Lowe, Wilson, Crowther, Tzeggai, Bishop, Varro: "Simultaneous Quantification of Six Human Cytokines in a Single Sample Using Microparticle-based Flow Cytometric Technology." in: Clinical chemistry, Vol. 45, Issue 9, pp. 1693-1694, (1999) (PubMed).

Oliver, Kettman, Fulton: "Multiplexed analysis of human cytokines by use of the FlowMetrix system." in: Clinical chemistry, Vol. 44, Issue 9, pp. 2057-60, (1998) (PubMed).

Bishop, Davis: "A flow cytometric immunoassay for beta2-microglobulin in whole blood." in: Journal of immunological methods, Vol. 210, Issue 1, pp. 79-87, (1998) (PubMed).

Fulton, McDade, Smith, Kienker, Kettman: "Advanced multiplexed analysis with the FlowMetrix system." in: Clinical chemistry, Vol. 43, Issue 9, pp. 1749-56, (1997) (PubMed).

Target Details

Target: IL-8, IL-1beta, IL-6, IL-10, TNF, IL-12p70

Sub Type: Cocktail