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IDE ELISA Kit

IDE Reactivity: Mouse Colorimetric Sandwich ELISA 3.125 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate
Catalog No. ABIN1570455
  • Target See all IDE ELISA Kits
    IDE (Insulin-Degrading Enzyme (IDE))
    Reactivity
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    3.125 pg/mL - 200 pg/mL
    Minimum Detection Limit
    3.125 pg/mL
    Application
    ELISA
    Purpose
    The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of IDE in mouse serum, plasma, tissue homogenates and other biological fluids.
    Sample Type
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of this index.
    Cross-Reactivity (Details)
    No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
    Sensitivity
    1.33 pg/mL
    Components
    • Pre-coated, ready to use 96-well strip plate
    • Standard (freeze dried)
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • TMB
    • Stop Solution
    • Wash Buffer (30X)
    • Plate sealer for 96 wells
    • Instruction manual
    Material not included
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Sample Volume
    100 μL
    Assay Time
    1 - 4.5 h
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Assay Procedure

    The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    Assay Precision
    • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
    • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
    • CV(%) = SD/meanX100
    • Intra-assay: CV<10%
    • Inter-assay: CV<12%
    Restrictions
    For Research Use only
  • Precaution of Use
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    Handling Advice
    The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
    Storage
    4 °C,-20 °C
    Storage Comment
    The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
    Expiry Date
    12 months
  • Target See all IDE ELISA Kits
    IDE (Insulin-Degrading Enzyme (IDE))
    Alternative Name
    IDE (IDE Products)
    Synonyms
    zgc:162603 ELISA Kit, IDE ELISA Kit, 1300012G03Rik ELISA Kit, 4833415K22Rik ELISA Kit, AA675336 ELISA Kit, AI507533 ELISA Kit, INSDEGM ELISA Kit, INSULYSIN ELISA Kit, insulin degrading enzyme ELISA Kit, insulin-degrading enzyme ELISA Kit, metallopeptidase ELISA Kit, IDE ELISA Kit, ide ELISA Kit, LOC591315 ELISA Kit, CC1G_01115 ELISA Kit, Bm1_26615 ELISA Kit, PTRG_10875 ELISA Kit, SJAG_01430 ELISA Kit, EBI_21700 ELISA Kit, VDBG_06702 ELISA Kit, LOAG_08082 ELISA Kit, PGTG_04982 ELISA Kit, Tsp_00424 ELISA Kit, Ide ELISA Kit
    Background
    Alternative name: Insulysin, Insulin Protease, Abeta-degrading protease, Insulinase
    Gene ID
    15925
    UniProt
    Q9JHR7
    Pathways
    SARS-CoV-2 Protein Interactome
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