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Perforin 1 ELISA Kit

PRF1 Reactivity: Mouse Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Catalog No. ABIN1572009
  • Target See all Perforin 1 (PRF1) ELISA Kits
    Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))
    Reactivity
    • 7
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.625 ng/mL - 40 ng/mL
    Minimum Detection Limit
    0.625 ng/mL
    Application
    ELISA
    Purpose
    The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PRF1 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of this index.
    Cross-Reactivity (Details)
    No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
    Sensitivity
    0.224 ng/mL
    Components
    • Pre-coated, ready to use 96-well strip plate
    • Standard (freeze dried)
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • TMB
    • Stop Solution
    • Wash Buffer (30X)
    • Plate sealer for 96 wells
    • Instruction manual
    Material not included
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Sample Volume
    100 μL
    Assay Time
    1 - 4.5 h
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Assay Procedure

    The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    Assay Precision
    • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
    • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
    • CV(%) = SD/meanX100
    • Intra-assay: CV<10%
    • Inter-assay: CV<12%
    Restrictions
    For Research Use only
  • Precaution of Use
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    Handling Advice
    The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
    Storage
    4 °C,-20 °C
    Storage Comment
    The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
    Expiry Date
    12 months
  • Target See all Perforin 1 (PRF1) ELISA Kits
    Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))
    Alternative Name
    PRF1 (PRF1 Products)
    Synonyms
    FLH2 ELISA Kit, HPLH2 ELISA Kit, P1 ELISA Kit, PFN1 ELISA Kit, PFP ELISA Kit, PRF1 ELISA Kit, Pfn ELISA Kit, Pfp ELISA Kit, Prf-1 ELISA Kit, Cyta ELISA Kit, RATCYTA ELISA Kit, LOC443187 ELISA Kit, perforin ELISA Kit, prf1 ELISA Kit, cytolysin ELISA Kit, perforin-1 ELISA Kit, perforin-1-like ELISA Kit, perforin 1 ELISA Kit, perforin 1 (pore forming protein) ELISA Kit, perforin ELISA Kit, perforin 1 L homeolog ELISA Kit, PRF1 ELISA Kit, Prf1 ELISA Kit, LOC443187 ELISA Kit, prf1 ELISA Kit, prf1.L ELISA Kit
    Background
    Alternative name: FLH2, HPLH2, P1, PFP, Pore Forming Protein, Cytolysin, Lymphocyte pore-forming protein
    Gene ID
    18646
    UniProt
    P10820
    Pathways
    Apoptosis, Caspase Cascade in Apoptosis
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