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IL-6 Receptor ELISA Kit

IL6R Reactivity: Human AA 20-358 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum, Urine
Catalog No. ABIN1672798
  • Target See all IL-6 Receptor (IL6R) ELISA Kits
    IL-6 Receptor (IL6R) (Interleukin 6 Receptor (IL6R))
    Binding Specificity
    AA 20-358
    Reactivity
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    31.2-2000 pg/mL
    Minimum Detection Limit
    31.2 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-6R alpha
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate), Urine
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: sf21
    Immunogen sequence: L20-D358
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: sf21
    Immunogen sequence: L20-D358
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plate
    Pre-coated
    Protocol
    human IL-6R alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-6R alpha has been precoated onto 96-well plates. Standards(sf21, L20-D358) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-6R alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-6R alpha amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human IL-6R alpha standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA, citrate) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-6R alpha standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 115, Standard deviation: 9.2, CV(%): 8
    • Sample 2: n=16, Mean(pg/ml): 768, Standard deviation: 26.11, CV(%): 3.4
    • Sample 3: n=16, Mean(pg/ml): 1558, Standard deviation: 60.76, CV(%): 3.9,
    • Sample 1: n=24, Mean(pg/ml): 124, Standard deviation: 10.54, CV(%): 8.5
    • Sample 2: n=24, Mean(pg/ml): 845, Standard deviation: 47.32, CV(%): 5.6
    • Sample 3: n=24, Mean(pg/ml): 1334, Standard deviation: 81.37, CV(%): 6.1
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all IL-6 Receptor (IL6R) ELISA Kits
    IL-6 Receptor (IL6R) (Interleukin 6 Receptor (IL6R))
    Alternative Name
    IL6R (IL6R Products)
    Synonyms
    IL6R ELISA Kit, il-6ra ELISA Kit, CD126 ELISA Kit, IL-6R-1 ELISA Kit, IL-6RA ELISA Kit, IL6Q ELISA Kit, IL6RA ELISA Kit, IL6RQ ELISA Kit, gp80 ELISA Kit, IL6R1 ELISA Kit, Il6ra ELISA Kit, IL-6Ralpha ELISA Kit, IL-6R ELISA Kit, Il6r ELISA Kit, interleukin 6 receptor ELISA Kit, interleukin 6 receptor, alpha ELISA Kit, IL6R ELISA Kit, il6r ELISA Kit, Il6r ELISA Kit, Il6ra ELISA Kit
    Background

    Background: IL6R alpha(IL6RA) also known as CD126 or IL6R, is a type I cytokine receptor. The IL6 receptor is a protein complex consisting of a IL-6 receptor subunit(IL6R) and interleukin 6 signal transducer Glycoprotein 130. IL6R also denotes the human gene encoding this subunit. Alternatively spliced transcript variants encoding distinct isoforms have been reported. IL6R subunit is also shared by many other cytokines. Interleukin-6 receptor has been shown to interact with Interleukin 6 and Ciliary neurotrophic factor. Ligand binding did not appear to affect IL6R dimerization status. IL6R dimerization occurs both on the cell surface and in solution.

    Synonyms: Interleukin 6 receptor ,Interleukin 6 receptor, isoform CRA_a ,IL6R ,hCG_1810911 ,

    Full Gene Name: interleukin 6 receptor

    Gene ID
    3570
    UniProt
    A0N0L5
    Pathways
    JAK-STAT Signaling, Autophagy, Growth Factor Binding, Cancer Immune Checkpoints
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