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SERPINA4 ELISA Kit

SERPINA4 Reactivity: Human AA 21-427 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN1672812
  • Target See all SERPINA4 ELISA Kits
    SERPINA4 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 4 (SERPINA4))
    Binding Specificity
    AA 21-427
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    156-10000 pg/mL
    Minimum Detection Limit
    156 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Kallistatin/Serpina4
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: Q21-P427
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: Q21-P427
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Belongs to the serpin family.

    Tissue Specificity: Expressed by the liver and secreted in plasma.

    Plate
    Pre-coated
    Protocol
    human Kallistatin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Kallistatin has been precoated onto 96-well plates. Standards(NSO, Q21-P427) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Kallistatin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Kallistatin amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Kallistatin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Kallistatin standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 1521, Standard deviation: 77.6, CV(%): 5.1
    • Sample 2: n=16, Mean(pg/ml): 3786, Standard deviation: 170.4, CV(%): 4.5
    • Sample 3: n=16, Mean(pg/ml): 6866, Standard deviation: 425.7, CV(%): 6.2,
    • Sample 1: n=24, Mean(pg/ml): 1637, Standard deviation: 104.8, CV(%): 6.4
    • Sample 2: n=24, Mean(pg/ml): 4242, Standard deviation: 237.6, CV(%): 5.6
    • Sample 3: n=24, Mean(pg/ml): 7128, Standard deviation: 499, CV(%): 7
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all SERPINA4 ELISA Kits
    SERPINA4 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 4 (SERPINA4))
    Alternative Name
    SERPINA4 (SERPINA4 Products)
    Synonyms
    KLKR ELISA Kit, Klk6 ELISA Kit, hK1 ELISA Kit, KAL ELISA Kit, KLST ELISA Kit, KST ELISA Kit, PI-4 ELISA Kit, PI4 ELISA Kit, kallistatin ELISA Kit, SERPINA4 ELISA Kit, DKFZp469E1737 ELISA Kit, KALps ELISA Kit, Klst ELISA Kit, kallikrein 1 ELISA Kit, serpin family A member 4 ELISA Kit, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4 ELISA Kit, serine (or cysteine) peptidase inhibitor, clade A, member 4, pseudogene 1 ELISA Kit, KLK1 ELISA Kit, SERPINA4 ELISA Kit, Serpina4 ELISA Kit, SPIA4 ELISA Kit, Serpina4-ps1 ELISA Kit
    Background

    Protein Function: Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein. .

    Background: Serpin peptidase inhibitor, clade A(alpha-1 antiproteinase, antitrypsin), member 4, also called Kallistatin, is a protein that in humans is encoded by the SERPINA4 gene. This gene is mapped to chromosome 14q32.13. Kallistatin is a serine proteinase inhibitor(serpin) and a heparin-binding protein. It is localized in vascular smooth muscle cells and endothelial cells of blood vessels, suggesting that it may be involved in the regulation of vascular function. It shows that kallistatin plays a role in neointima hyperplasia. The potential role of kallistatin was investigated in angiogenesis in vitro and in vivo, and presented results demonstrating a novel role of the protein in the inhibition of angiogenesis and tumor growth.

    Synonyms: Kallistatin,Kallikrein inhibitor,Peptidase inhibitor 4,PI-4,Serpin A4,SERPINA4,KST, PI4,

    Full Gene Name: Kallistatin

    Cellular Localisation: Secreted.
    Gene ID
    5267
    UniProt
    P29622
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