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MIA ELISA Kit

MIA Reactivity: Human AA 25-131 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Saliva, Serum
Catalog No. ABIN1672853
  • Target See all MIA ELISA Kits
    MIA (Melanoma Inhibitory Activity (MIA))
    Binding Specificity
    AA 25-131
    Reactivity
    • 5
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    15.6-1000 pg/mL
    Minimum Detection Limit
    15.6 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MIA
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: E.coli
    Immunogen sequence: G25-131Q
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: E.coli
    Immunogen sequence: G25-131Q
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Belongs to the MIA/OTOR family.

    Tissue Specificity: All malignant melanoma cell lines tested and infrequently in glioma cell lines.

    Plate
    Pre-coated
    Protocol
    human MIA ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MIA has been precoated onto 96-well plates. Standards(E.coli, G25-131Q) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MIA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MIA amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human MIA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or saliva to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human MIA standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 139, Standard deviation: 5.42, CV(%): 3.9
    • Sample 2: n=16, Mean(pg/ml): 325, Standard deviation: 16.25, CV(%): 5
    • Sample 3: n=16, Mean(pg/ml): 642, Standard deviation: 36.6, CV(%): 5.7,
    • Sample 1: n=24, Mean(pg/ml): 157, Standard deviation: 7.54, CV(%): 4.8
    • Sample 2: n=24, Mean(pg/ml): 368, Standard deviation: 22.45, CV(%): 6.1
    • Sample 3: n=24, Mean(pg/ml): 667, Standard deviation: 46, CV(%): 6.9
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all MIA ELISA Kits
    MIA (Melanoma Inhibitory Activity (MIA))
    Alternative Name
    MIA (MIA Products)
    Synonyms
    CD-RAP ELISA Kit, Mia1 ELISA Kit, Cdrap ELISA Kit, zgc:194157 ELISA Kit, mia ELISA Kit, CDRAP ELISA Kit, Fdp ELISA Kit, MIA ELISA Kit, MIAL ELISA Kit, melanoma inhibitory activity ELISA Kit, otoraplin ELISA Kit, cilia and flagella associated protein 100 ELISA Kit, MIA ELISA Kit, Mia ELISA Kit, mia ELISA Kit, Otor ELISA Kit, CFAP100 ELISA Kit
    Background

    Protein Function: Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.

    Background: Melanoma Inhibiting Activity(MIA), also known as cartilage-derived retinoic acid-sensitive protein(CD-RAP), is an approximately 11-15 kDa protein that is expressed as a noncovalent homodimer. MIA is structurally related to OTOR/Otoraplin and MIA-2 in a small family of secreted proteins with one SH3 domain(1-3). And the MIA gene was mapped to 19q13.32-q13.33 by fluorescence in situ hybridization. Beside, It is a marker for malignant melanoma. MIA functions as a chemoattractant for mesenchymal stem cells and enhances their BMP-2 and TGF-beta3 induced differentiation into chondrocytes . MIA-deficient mice exhibit delayed chondrocyte differentiation but enhanced chondrocyte proliferation and cartilage repair .

    Synonyms: Melanoma-derived growth regulatory protein,Melanoma inhibitory activity protein,MIA,

    Full Gene Name: Melanoma-derived growth regulatory protein

    Cellular Localisation: Secreted.
    Gene ID
    8190
    UniProt
    Q16674
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