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FSTL1 ELISA Kit

FSTL1 Reactivity: Human AA 1-308 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN1672869
  • Target See all FSTL1 ELISA Kits
    FSTL1 (Follistatin-Like 1 (FSTL1))
    Binding Specificity
    AA 1-308
    Reactivity
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    312-20000 pg/mL
    Minimum Detection Limit
    312 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human FSTL1
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: M1-I308
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: M1-I308
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Contains 2 EF-hand domains.

    Tissue Specificity: Overexpressed in synovial tissues from rheumatoid arthritis. .

    Plate
    Pre-coated
    Protocol
    human FSTL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FSTL1 has been precoated onto 96-well plates. Standards(NSO, M1-I308) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FSTL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human FSTL1 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human FSTL1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human FSTL1 standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(ng/ml): 1.34, Standard deviation: 0.056, CV(%): 4.2
    • Sample 2: n=16, Mean(ng/ml): 7.2, Standard deviation: 0.382, CV(%): 5.3
    • Sample 3: n=16, Mean(ng/ml): 13.9, Standard deviation: 0.89, CV(%): 6.4,
    • Sample 1: n=24, Mean(ng/ml): 1.25, Standard deviation: 0.094, CV(%): 7.5
    • Sample 2: n=24, Mean(ng/ml): 7.5, Standard deviation: 0.48, CV(%): 6.4
    • Sample 3: n=24, Mean(ng/ml): 14.4, Standard deviation: 1.123, CV(%): 7.8
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Wang, Li, Xu, Tao, Zhu, Sun, Fan, Zhang, Dong, Yu: "Follistatin-like protein 1: a serum biochemical marker reflecting the severity of joint damage in patients with osteoarthritis." in: Arthritis research & therapy, Vol. 13, Issue 6, pp. R193, (2012) (PubMed).

  • Target See all FSTL1 ELISA Kits
    FSTL1 (Follistatin-Like 1 (FSTL1))
    Alternative Name
    FSTL1 (FSTL1 Products)
    Synonyms
    FRP ELISA Kit, FSL1 ELISA Kit, MIR198 ELISA Kit, AI316791 ELISA Kit, AW107808 ELISA Kit, Fstl ELISA Kit, TSC-36 ELISA Kit, Frp ELISA Kit, fstl1 ELISA Kit, wu:fc75h02 ELISA Kit, zgc:110703 ELISA Kit, FLIK ELISA Kit, FSTL1 ELISA Kit, DKFZp469P101 ELISA Kit, follistatin like 1 ELISA Kit, follistatin-like 1 ELISA Kit, follistatin-like 1a ELISA Kit, Follistatin-related protein 1 ELISA Kit, follistatin-related protein 1 ELISA Kit, follistatin like 1 L homeolog ELISA Kit, FSTL1 ELISA Kit, Fstl1 ELISA Kit, fstl1a ELISA Kit, fstl1 ELISA Kit, LOC101909195 ELISA Kit, fstl1.L ELISA Kit
    Background

    Protein Function: May modulate the action of some growth factors on cell proliferation and differentiation. Binds heparin (By similarity). .

    Background: Follistatin-related protein 1, also called FSTL1 or FRP is a protein that in humans is encoded by the FSTL1 gene. FSTL1 gene is mapped to chromosome 3q13.33 based on an alignment of FSTL1 sequence with the genomic sequence(GRCh37). This gene encodes a protein with similarity to follistatin, an activin-binding protein. It contains an FS module, a follistatin-like sequence containing 10 conserved cysteine residues. This gene product is thought to be an autoantigen associated with rheumatoid arthritis. This gene may modulate the action of some growth factors on cell proliferation and differentiation. It was suggested that fibroblast-secreted FSTL1 acts on macrophages to increase TNF and IL1B production and IL6 activity.

    Synonyms: Follistatin-related protein 1,Follistatin-like protein 1,FSTL1,FRP,

    Full Gene Name: Follistatin-related protein 1

    Cellular Localisation: Secreted.
    Gene ID
    11167
    UniProt
    Q12841
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