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Neurotrophin 4 ELISA Kit

NTF4 Reactivity: Human AA 81-210 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Serum
Catalog No. ABIN1706065
  • Target See all Neurotrophin 4 (NTF4) ELISA Kits
    Neurotrophin 4 (NTF4)
    Binding Specificity
    AA 81-210
    Reactivity
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    31.2-2000 pg/mL
    Minimum Detection Limit
    31.2 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human NT-4
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: sf21
    Immunogen sequence: G81-A210
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: sf21
    Immunogen sequence: G81-A210
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Tissue Specificity: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues.

    Plate
    Pre-coated
    Protocol
    human NT-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for NT-4 has been precoated onto 96-well plates. Standards(sf21, G81-A210) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NT-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human NT-4 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human NT-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human NT-4 standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 248, Standard deviation: 11.16, CV(%): 4.5
    • Sample 2: n=16, Mean(pg/ml): 745, Standard deviation: 38, CV(%): 5.1
    • Sample 3: n=16, Mean(pg/ml): 1243, Standard deviation: 78.31, CV(%): 6.3,
    • Sample 1: n=24, Mean(pg/ml): 280, Standard deviation: 14.84, CV(%): 5.3
    • Sample 2: n=24, Mean(pg/ml): 821, Standard deviation: 46, CV(%): 5.6
    • Sample 3: n=24, Mean(pg/ml): 1402, Standard deviation: 96.74, CV(%): 6.9
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Li, Jin, Wang, Li, Liu, Chang, Wang, Liu: "Live attenuated Salmonella displaying HIV-1 10E8 epitope on fimbriae: systemic and mucosal immune responses in BALB/c mice by mucosal administration." in: Scientific reports, Vol. 6, pp. 29556, (2018) (PubMed).

    Sun, Zhang, Li, Wang, Ma, Sun, Liu, Wang, Zhou: "Interaction between neurotrophin 4 and gonadotrophin in bovine oviducts." in: Theriogenology, Vol. 78, Issue 1, pp. 39-48, (2012) (PubMed).

  • Target See all Neurotrophin 4 (NTF4) ELISA Kits
    Neurotrophin 4 (NTF4)
    Alternative Name
    NTF4 (NTF4 Products)
    Synonyms
    NTF5 ELISA Kit, GLC10 ELISA Kit, GLC1O ELISA Kit, NT-4 ELISA Kit, NT-4/5 ELISA Kit, NT-5 ELISA Kit, NT4 ELISA Kit, NT5 ELISA Kit, NT4P ELISA Kit, Ntf5 ELISA Kit, 2900040K06Rik ELISA Kit, AI462899 ELISA Kit, NT4/5 ELISA Kit, Ntf-5 ELISA Kit, Ntf4 ELISA Kit, neurotrophin 4 ELISA Kit, neurotrophin 5 ELISA Kit, NTF4 ELISA Kit, Ntf4 ELISA Kit, ntf4 ELISA Kit, Ntf5 ELISA Kit
    Background

    Protein Function: Target-derived survival factor for peripheral sensory sympathetic neurons.

    Background: Neurotrophin-4(NT-4), also known as neurotrophin-5(NT-5), is a protein that in humans is encoded by the NTF4 gene. Human NT-4 as well as a human NT-4 pseudogene colocalize to chromosome 19 band q13.3. NT-4 is a member of a family of neurotrophic factors, the neurotrophins, that control survival and differentiation of vertebrate neurons(2-4). NT-4 is a neurotrophic factor that signals predominantly through the TrkB receptor tyrosine kinase. NT4 plays a physiological role essential for hippocampus- and amygdala-dependent long-term memory and hippocampal long-lasting LTP and that NT4 may be useful in the therapy of acquired disorders of learning and memory.

    Synonyms: Neurotrophin-4,NT-4,Neurotrophin-5,NT-5,Neutrophic factor 4,NTF4,NTF5,

    Full Gene Name: Neurotrophin-4

    Cellular Localisation: Secreted.
    Gene ID
    4909
    UniProt
    P34130
    Pathways
    RTK Signaling
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