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RANKL ELISA Kit

TNFSF11 Reactivity: Human AA 64-245 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN1706067
  • Target See all RANKL (TNFSF11) ELISA Kits
    RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))
    Binding Specificity
    AA 64-245
    Reactivity
    • 5
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    78-5000 pg/mL
    Minimum Detection Limit
    78 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TNFSF11/RANKL
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: G64-D245
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: G64-D245
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Tissue Specificity: Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid.

    Plate
    Pre-coated
    Protocol
    human TNFSF11 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFSF11 has been precoated onto 96-well plates. Standards(NSO, G64-D245) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFSF11 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNFSF11 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL human TNFSF11 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human TNFSF11 standard solution and each sample is measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 624, Standard deviation: 20.6, CV(%): 3.3
    • Sample 2: n=16, Mean(pg/ml): 1302, Standard deviation: 66.4, CV(%): 5.1
    • Sample 3: n=16, Mean(pg/ml): 2684, Standard deviation: 123.5, CV(%): 4.6,
    • Sample 1: n=24, Mean(pg/ml): 714, Standard deviation: 28.56, CV(%): 4
    • Sample 2: n=24, Mean(pg/ml): 1428, Standard deviation: 92.82, CV(%): 6.5
    • Sample 3: n=24, Mean(pg/ml): 2710, Standard deviation: 154.5, CV(%): 5.7
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Zhao, Ning, Wang, Li, Qiao, Yao, Qin: "Calcitonin gene-related peptide inhibits osteolytic factors induced by osteoblast in co-culture system with breast cancer." in: Cell biochemistry and biophysics, Vol. 70, Issue 2, pp. 1097-104, (2014) (PubMed).

    Xu, Wang, Lu, Xu: "Osteoprotegerin and RANKL in the pathogenesis of rheumatoid arthritis-induced osteoporosis." in: Rheumatology international, Vol. 32, Issue 11, pp. 3397-403, (2012) (PubMed).

    Chen, Li, Liu, Wang, Xie, Liu, Hou, Chang, Du: "Canonical Wnt signaling is required for Panax notoginseng saponin-mediated attenuation of the RANKL/OPG ratio in bone marrow stromal cells during osteogenic differentiation." in: Phytomedicine : international journal of phytotherapy and phytopharmacology, Vol. 19, Issue 11, pp. 1029-34, (2012) (PubMed).

    Tang, Zhang, Tang, Qi, Jiang: "Hypoxia induces RANK and RANKL expression by activating HIF-1? in breast cancer cells." in: Biochemical and biophysical research communications, Vol. 408, Issue 3, pp. 411-6, (2011) (PubMed).

    Tang, Sun, Li, Zhou, Yin, Zhou: "Porphyromonas endodontalis lipopolysaccharides induce RANKL by mouse osteoblast in a way different from that of Escherichia coli lipopolysaccharide." in: Journal of endodontics, Vol. 37, Issue 12, pp. 1653-8, (2011) (PubMed).

  • Target See all RANKL (TNFSF11) ELISA Kits
    RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))
    Alternative Name
    TNFSF11 (TNFSF11 Products)
    Synonyms
    CD254 ELISA Kit, ODF ELISA Kit, OPGL ELISA Kit, OPTB2 ELISA Kit, RANKL ELISA Kit, TRANCE ELISA Kit, hRANKL2 ELISA Kit, sOdf ELISA Kit, TNFSF11 ELISA Kit, Ly109l ELISA Kit, OPG ELISA Kit, Trance ELISA Kit, cd254 ELISA Kit, odf ELISA Kit, opgl ELISA Kit, optb2 ELISA Kit, rankl ELISA Kit, sodf ELISA Kit, tnfsf11 ELISA Kit, trance ELISA Kit, TNF superfamily member 11 ELISA Kit, tumor necrosis factor (ligand) superfamily, member 11 ELISA Kit, TNF receptor superfamily member 11b ELISA Kit, TNF superfamily member 11 L homeolog ELISA Kit, TNFSF11 ELISA Kit, Tnfsf11 ELISA Kit, TNFRSF11B ELISA Kit, tnfsf11.L ELISA Kit
    Background

    Protein Function: Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. .

    Background: Receptor activator of nuclear factor kappa-B ligand(RANKL), also known as tumor necrosis factor ligand superfamily member 11(TNFSF11), is a protein that in humans is encoded by the TNFSF11 gene. This gene encodes a member of the tumor necrosis factor(TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This gene is mapped to chromosome13q14.11. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. This gene may play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.

    Synonyms: Tumor necrosis factor ligand superfamily member 11,Osteoclast differentiation factor,ODF,Osteoprotegerin ligand,OPGL,Receptor activator of nuclear factor kappa-B ligand,RANKL,TNF-related activation-induced cytokine,TRANCE,CD254,Tumor necrosis factor ligand superfamily member 11, membrane form,Tumor necrosis factor ligand superfamily member 11, soluble form,TNFSF11,OPGL, RANKL, TRANCE,

    Full Gene Name: Tumor necrosis factor ligand superfamily member 11

    Cellular Localisation: Isoform 1: Cell membrane, Single-pass type II membrane protein.
    Gene ID
    8600
    UniProt
    O14788
    Pathways
    NF-kappaB Signaling
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