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CCL16 ELISA Kit

CCL16 Reactivity: Human AA 24-120 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN1706078
  • Target See all CCL16 ELISA Kits
    CCL16 (Chemokine (C-C Motif) Ligand 16 (CCL16))
    Binding Specificity
    AA 24-120
    Reactivity
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    7.8-500 pg/mL
    Minimum Detection Limit
    7.8 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CCL16/HCC-4
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: E.coli
    Immunogen sequence: Q24-Q120
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: E.coli
    Immunogen sequence: Q24-Q120
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.

    Tissue Specificity: Mainly expressed in liver, also found in spleen and thymus. Highly expressed in LPS- and IFN-gamma- activated monocytes, weakly in some lymphocytes, including natural killer cells, gamma-delta T-cells, and some T-cell clones.

    Plate
    Pre-coated
    Protocol
    human CCL16 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL16 has been precoated onto 96-well plates. Standards(E.coli, Q24-Q120) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL16 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL16 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human CCL16 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human CCL16 standard solution and each sample is measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 25, Standard deviation: 0.80, CV(%): 3.2
    • Sample 2: n=16, Mean(pg/ml): 76, Standard deviation: 4.26, CV(%): 5.6
    • Sample 3: n=16, Mean(pg/ml): 237, Standard deviation: 13.98, CV(%): 5.9,
    • Sample 1: n=24, Mean(pg/ml): 26, Standard deviation: 1.51, CV(%): 5.8
    • Sample 2: n=24, Mean(pg/ml): 81, Standard deviation: 5.02, CV(%): 6.2
    • Sample 3: n=24, Mean(pg/ml): 242, Standard deviation: 16.21, CV(%): 6.7
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all CCL16 ELISA Kits
    CCL16 (Chemokine (C-C Motif) Ligand 16 (CCL16))
    Alternative Name
    CCL16 (CCL16 Products)
    Synonyms
    CKb12 ELISA Kit, HCC-4 ELISA Kit, ILINCK ELISA Kit, LCC-1 ELISA Kit, LEC ELISA Kit, LMC ELISA Kit, Mtn-1 ELISA Kit, NCC-4 ELISA Kit, NCC4 ELISA Kit, SCYA16 ELISA Kit, SCYL4 ELISA Kit, C-C motif chemokine ligand 16 ELISA Kit, CCL16 ELISA Kit, Ccl16 ELISA Kit
    Background

    Protein Function: Shows chemotactic activity for lymphocytes and monocytes but not neutrophils. Also shows potent myelosuppressive activity, suppresses proliferation of myeloid progenitor cells. Recombinant SCYA16 shows chemotactic activity for monocytes and THP-1 monocytes, but not for resting lymphocytes and neutrophils. Induces a calcium flux in THP-1 cells that were desensitized by prior expression to RANTES.

    Background: Chemokine(C-C motif) ligand 16(CCL16) is a small cytokine belonging to the CC chemokine family that is known under several pseudonyms, including Liver-expressed chemokine(LEC) and Monotactin-1(MTN-1). This chemokine is expressed by the liver, thymus, and spleen and is chemoattractive for monocytes and lymphocytes. Cellular expression of CCL16 can be strongly induced in monocytes by IL-10, IFN-gamma and bacterial lipopolysaccharide. Its gene is located on chromosome 17, in humans, among a cluster of other CC chemokines. CCL16 elicits its effects on cells by interacting with cell surface chemokine receptors such as CCR1, CCR2, CCR5 and CCR8.

    Synonyms: C-C motif chemokine 16,Chemokine CC-4,HCC-4,Chemokine LEC,IL-10-inducible chemokine,LCC-1,Liver-expressed chemokine,Lymphocyte and monocyte chemoattractant,LMC,Monotactin-1,MTN-1,NCC-4,Small-inducible cytokine A16,CCL16,ILINCK, NCC4, SCYA16,

    Full Gene Name: C-C motif chemokine 16

    Cellular Localisation: Secreted.
    Gene ID
    6360
    UniProt
    O15467
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