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SensoLyte® 520 MMP-9 Assay Kit

FRET Fluorometric
Catalog No. ABIN1882528
  • Target See all MMP 9 (MMP9) Kits
    MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))
    Detection Method
    Fluorometric
    Application
    Fluorescence Resonance Energy Transfer Microscopy (FRET)
    Brand
    SensoLyte®
    Characteristics
    SensoLyte® 520 MMP-9 Assay Kit uses a 5-FAM (fluorophore) and QXL520™ (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. In an intact FRETpeptide, the fluorescence of 5-FAM is quenched by QXL520™. Upon the cleavage of the FRET peptide by MMP-9, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL520™ based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity.
    Top Product
    Discover our top product MMP9 ELISA Kit
  • Comment

    FRET-based Assay Kit

    Restrictions
    For Research Use only
  • Handling Advice
    Protect Components A and B from light.
    Storage
    -20 °C
    Storage Comment
    Store all components at -20 °C. Components D and E can be stored at 4 °C for convenience.
  • Pifer, Maerz, Baker, Anderson et al.: "Matrix metalloproteinase content and activity in low-platelet, low-leukocyte and high-platelet, high-leukocyte platelet rich plasma (PRP) and the biologic response to PRP by human ligament ..." in: The American journal of sports medicine, Vol. 42, Issue 5, pp. 1211-8, (2014) (PubMed).

    Neill, Painter, Buraschi, Owens, Lisanti, Schaefer, Iozzo: "Decorin antagonizes the angiogenic network: concurrent inhibition of Met, hypoxia inducible factor 1α, vascular endothelial growth factor A, and induction of thrombospondin-1 and TIMP3." in: The Journal of biological chemistry, Vol. 287, Issue 8, pp. 5492-506, (2012) (PubMed).

    Inoue, Mashimo, Funamizu, Yonekura, Horiguchi, Shimojo, Kohno, Okamoto, Hata, Suzuki: "Association of the MMP9 gene with childhood cedar pollen sensitization and pollinosis." in: Journal of human genetics, Vol. 57, Issue 3, pp. 176-83, (2012) (PubMed).

    Spolidoro, Putignano, Munafò, Maffei, Pizzorusso: "Inhibition of matrix metalloproteinases prevents the potentiation of nondeprived-eye responses after monocular deprivation in juvenile rats." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 22, Issue 3, pp. 725-34, (2012) (PubMed).

    Wigner, Kulkarni, Yakavonis, Young, Tinsley, Meeks, Einhorn, Gerstenfeld: "Urine matrix metalloproteinases (MMPs) as biomarkers for the progression of fracture healing." in: Injury, Vol. 43, Issue 3, pp. 274-8, (2012) (PubMed).

    Yuan, Johnson, Chen, Wei, Pellecchia: "A novel pharmacophore model for the design of anthrax lethal factor inhibitors." in: Chemical biology & drug design, Vol. 76, Issue 3, pp. 263-8, (2010) (PubMed).

    Allington, Galliher-Beckley, Schiemann: "Activated Abl kinase inhibits oncogenic transforming growth factor-beta signaling and tumorigenesis in mammary tumors." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 23, Issue 12, pp. 4231-43, (2009) (PubMed).

    Johnson, Chen, Harbach, Sabet, Savinov, Cotton, Strongin, Guiney, Pellecchia: "Rhodanine derivatives as selective protease inhibitors against bacterial toxins." in: Chemical biology & drug design, Vol. 71, Issue 2, pp. 131-9, (2008) (PubMed).

    Soehnlein, Oehmcke, Ma, Rothfuchs, Frithiof, van Rooijen, Mörgelin, Herwald, Lindbom: "Neutrophil degranulation mediates severe lung damage triggered by streptococcal M1 protein." in: The European respiratory journal, Vol. 32, Issue 2, pp. 405-12, (2008) (PubMed).

    Johnson, Chen, Pellecchia: "A high-throughput screening approach to anthrax lethal factor inhibition." in: Bioorganic chemistry, Vol. 35, Issue 4, pp. 306-12, (2007) (PubMed).

  • Target
    MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))
    Alternative Name
    MMP-9 (MMP9 Products)
    Synonyms
    CLG4B Kit, GELB Kit, MANDP2 Kit, MMP-9 Kit, AW743869 Kit, B/MMP9 Kit, Clg4b Kit, pro-MMP-9 Kit, mmp-9 Kit, fgMMP-9 Kit, mmp9 Kit, ZFMMP-9 Kit, wu:fb02g06 Kit, wu:fb07b05 Kit, wu:fi98c09 Kit, wu:fj05a08 Kit, zgc:64165 Kit, clg4b Kit, gelb Kit, mandp2 Kit, matrix metallopeptidase 9 Kit, collagenase Kit, matrix metalloproteinase 9 Kit, matrix metallopeptidase 9 S homeolog Kit, MMP9 Kit, RB3913 Kit, BPSS0666 Kit, Sbal_3732 Kit, Bcer98_0486 Kit, Shew185_0630 Kit, Shal_3056 Kit, Sbal195_0657 Kit, Lbys_3550 Kit, Palpr_2084 Kit, Mmp9 Kit, mmp9 Kit, mmp9.S Kit
    Background
    MMP-9 (gelatinase-B, collagenase-IV) is involved in quite a few diseases such as a cancer, angiogenesis, alopecia and metastasis.
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