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SensoLyte® AFC Plasmin Activity Assay Kit

Act, FRET Fluorometric
Catalog No. ABIN1882541
  • Target See all Plasmin Kits
    Plasmin (Plasmin/fibrinolysin (PL/Fbn))
    Detection Method
    Fluorometric
    Application
    Activation (Act), Fluorescence Resonance Energy Transfer Microscopy (FRET)
    Brand
    SensoLyte®
    Characteristics
    The SensoLyte® AFC Plasmin Activity Assay Kit provides a convenient assay for high throughput screening of plasmin inhibitors and inducers or for continuous assay of plasmin activity. The assay utilizes a fluorogenic peptide, which upon enzyme cleavage releases the AFC (7-amido-4-trifluoromethylcoumarin) fluorophore. The fluorescent signal can be measured at Ex/ Em =380 nm/500 nm. This assay can detect picogram level of plasmin activity.
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    Discover our top product Plasmin ELISA Kit
  • Comment

    FRET-based Assay Kit

    Restrictions
    For Research Use only
  • Storage
    -80 °C
    Storage Comment
    Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Protect Components A and B from light and moisture. Components D and F can be stored at room temperature for convenience.
  • Portelli, Siedlinski, Stewart, Postma, Nieuwenhuis, Vonk, Nurnberg, Altmuller, Moffatt, Wardlaw, Parker, Connolly, Koppelman, Sayers: "Genome-wide protein QTL mapping identifies human plasma kallikrein as a post-translational regulator of serum uPAR levels." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 28, Issue 2, pp. 923-34, (2014) (PubMed).

    Park, Oh, Kim, Cho, Kim: "Identification of a specific haptoglobin C-terminal fragment in arthritic synovial fluid and its effect on interleukin-6 expression." in: Immunology, Vol. 140, Issue 1, pp. 133-41, (2013) (PubMed).

    Stewart, Nijmeh, Brightling, Sayers: "uPAR regulates bronchial epithelial repair in vitro and is elevated in asthmatic epithelium." in: Thorax, Vol. 67, Issue 6, pp. 477-87, (2012) (PubMed).

    Tudpor, Laínez, Kwakernaak, Kovalevskaya, Verkaart, van Genesen, van der Kemp, Navis, Bindels, Hoenderop: "Urinary plasmin inhibits TRPV5 in nephrotic-range proteinuria." in: Journal of the American Society of Nephrology : JASN, Vol. 23, Issue 11, pp. 1824-34, (2012) (PubMed).

  • Target
    Plasmin (Plasmin/fibrinolysin (PL/Fbn))
    Alternative Name
    Plasmin (Plasmin Products)
    Synonyms
    plasminogen Kit, PLG Kit
    Background
    Plasmin belongs to the family of serine proteases. It plays a key role in fibrinolysis by dissolving fibrin in blood clots. Besides fibrinolysis, plasmin is also involved in such physiological and pathological processes as wound healing, liver repair, and the maintenance of liver homeostasis.
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