SensoLyte® Green Sirt1 Assay Kit

Catalog No. ABIN1882554

Product Details

Target: SIRT1 (Sirtuin 1 (SIRT1))

Detection Method: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Brand: SensoLyte®

Characteristics: The SensoLyte® Green SIRT1 Assay Kit provides a convenient, two-step homogeneous procedure for measuring sirtuin 1 activity using a fluorogenic substrate. In the first step, an acetylated substrate is incubated with SIRT1 containing samples. Deacetylation of substrate first sensitizes it, so that, in the second step, mixing with the SIRT developer generates a fluorophore, which can then be detected at Ex/Em= 490 nm/520 nm. This kit can be used for high throughput screening of SIRT1 inhibitors/activators. The long wavelength fluorescence of SIRT1 substrate is less interfered by the autofluorescence of cell components and test compounds. Assays are performed in a standard 96-well format.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Aliquot as needed to avoid freeze-thaw cycles. Protect Components A and B from light and from moisture.

Storage: -80 °C

Storage Comment: Store all kit components, except Component C, at -20 °C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience.

References

Li, Feng, Xing, Wang, Du, Xu, Cao, Wang, Fan, Liu, Fan: "Radioprotective and antioxidant effect of resveratrol in hippocampus by activating Sirt1." in: International journal of molecular sciences, Vol. 15, Issue 4, pp. 5928-39, (2014) (PubMed).

Target Details

Target: SIRT1 (Sirtuin 1 (SIRT1))

Alternative Name: Sirt1 (SIRT1 Products)

Synonyms: sirtuin 1 Kit, AA673258 Kit, SIR2L1 Kit, Sir2 Kit, Sir2a Kit, Sir2alpha Kit, sir2l1 Kit, sirtuin Kit, SIR2 Kit, sirtuin 1 Kit, sirtuin 1 L homeolog Kit, SIRT1 Kit, SRT1 Kit, Sirt1 Kit, sirt1.L Kit

Background: Histone deacetylase (HDAC) enzymes act as transcriptional repressors of genes through the deacetylation of lysine residues on histone proteins. Sirtuins (SIRTs) are a unique class of deacetylases (class III HDACs) that require NAD as a cofactor. Sirtuin 1 (SIRT1) is involved in regulation of tumor suppressor p53 and inhibition of apoptosis. It also represents a target for treatment of age-related diseases and type II diabetes.