SensoLyte® 520 TACE (a-Secretase) Activity Assay Kit

Catalog No. ABIN1882558

Product Details

Target: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Reactivity: Human

Detection Method: Fluorometric

Application: Activation (Act), Fluorescence Resonance Energy Transfer Microscopy (FRET)

Brand: SensoLyte®

Characteristics: SensoLyte® 520 TACE Activity Assay Kit uses a 5-FAM (fluorophore) and QXL™ 520 (quencher) labeled FRET peptide substrate for continuous measurement of enzyme activity. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL™ 520. Upon cleavage of the FRET peptide by the active enzyme, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL™ 520 based FRET peptide has less interference from the autofluorescence of test compounds and cellular components and provides better assay sensitivity. The kit can be used to detect the activity of TACE or screen for TACE inhibitors. The assays are performed in a convenient 96-well format.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Protect Components A and B from light and from moisture.

Storage: -20 °C

Storage Comment: Store all kit components at -20 °C. Components C and E can be stored at room temperature for convenience.

References

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Target Details

Target: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Alternative Name: TACE (ADAM17 Products)

Synonyms: ADAM18 Kit, CD156B Kit, CSVP Kit, NISBD Kit, TACE Kit, CD156b Kit, Tace Kit, adam17 Kit, si:dkey-81b15.2 Kit, adam18 Kit, cd156b Kit, csvp Kit, tace Kit, fc20b01 Kit, wu:fc20b01 Kit, zgc:63886 Kit, ADAM metallopeptidase domain 17 Kit, a disintegrin and metallopeptidase domain 17 Kit, ADAM metallopeptidase domain 17b Kit, ADAM metallopeptidase domain 17 L homeolog Kit, ADAM metallopeptidase domain 17a Kit, ADAM17 Kit, Adam17 Kit, adam17b Kit, adam17.L Kit, adam17a Kit

Background: TACE (tumor necrosis factor-alpha converting enzyme), alpha-secretase, or ADAM17 is a member of the ADAM family of proteases that contain both disintegrin and metalloprotease (catalytic) domains. This enzyme is involved in sperm-egg binding and fusion, muscle cell fusion, neurogenesis, modulation of Notch receptor and ligand processing, and processing of the pro-inflammatory cytokine, TNFalpha. TACE is currently being explored as a target for anti-inflammatory drugs.