IGFBP2 ELISA Kit

Catalog No. ABIN1889294

Product Details IGFBP2 ELISA Kit

Target: IGFBP2 (Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2))

Binding Specificity: AA 40-328

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IGFBP2

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: E40-Q328

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: E40-Q328

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Contains 1 IGFBP N-terminal domain.

Plate: Pre-coated

Protocol: human IGFBP2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP2 has been precoated onto 96-well plates. Standards(NSO, E40-Q328) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IGFBP2 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human IGFBP2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin or EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IGFBP2 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 608, Standard deviation: 27.97, CV(%): 4.6
• Sample 2: n=16, Mean(pg/ml): 1555, Standard deviation: 80.86, CV(%): 5.2
• Sample 3: n=16, Mean(pg/ml): 2836, Standard deviation: 167.3, CV(%): 5.9,
• Sample 1: n=24, Mean(pg/ml): 715, Standard deviation: 44.33, CV(%): 6.2
• Sample 2: n=24, Mean(pg/ml): 1938, Standard deviation: 133.7, CV(%): 6.9
• Sample 3: n=24, Mean(pg/ml): 3147, Standard deviation: 229.7, CV(%): 7.3

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for IGFBP2

Target: IGFBP2 (Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2))

Alternative Name: IGFBP2 (IGFBP2 Products)

Background:

Protein Function: Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. .

Background: Insulin-like growth factor-binding protein 2(IGFBP2), also called IBP2, is a protein that in humans is encoded by the IGFBP2 gene. It is mapped to 2q35. By structural analysis, it showed that the IGFBP2 gene consists of 4 exons with 3 introns of lengths 27.0, 1.0, and 1.9 kb. IGFBP2 secreted by metastatic cells recruits endothelia by modulating IGF1 mediated activation of the IGF type-I receptor on endothelial cells. The IGFBP2/IGF1/IGF1R and GAS6/MERTK signaling pathways are regulators of cancer-mediated endothelial recruitment.

Synonyms: Insulin-like growth factor-binding protein 2,IBP-2,IGF-binding protein 2,IGFBP-2,IGFBP2,BP2, IBP2,

Full Gene Name: Insulin-like growth factor-binding protein 2

Cellular Localisation: Secreted.

Gene ID: 3485

UniProt: P18065

Pathways: Myometrial Relaxation and Contraction, Growth Factor Binding, Activated T Cell Proliferation