ESM1 ELISA Kit (Endothelial Cell-Specific Molecule 1)

Details for Product ESM1 ELISA Kit No. ABIN1889318, Supplier: Log in to see
Antigen
  • ESM1
  • zgc:153027
  • 0610042H23Rik
  • AV004503
  • ESM-1
  • Pg25
  • endocan
  • endothelial cell-specific molecule 1
  • ESM1
  • esm1
  • Esm1
Alternatives
Human ESM1 ELISA Kit
Epitope
AA 20-184
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
17
10
7
1
1
1
1
1
Method Type
Sandwich ELISA
Detection Range
23.4-1500 pg/mL
Minimum Detection Limit
23.4 pg/mL
Application
ELISA
Options
Supplier
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Supplier Product No.
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Purpose Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse ESM1/Endocan
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Analytical Method Quantitative
Detection Method Colorimetric
Immunogen Expression system for standard: NSO
Immunogen sequence: W20-R184
Specificity Expression system for standard: NSO
Immunogen sequence: W20-R184
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Alternative Name ESM1 (ESM1 ELISA Kit Abstract)
Background

Protein Function: Involved in angiogenesis, promotes angiogenic sprouting. May have potent implications in lung endothelial cell-leukocyte interactions (By similarity). .

Background: Endothelial cell-specific molecule 1, also known as Endocan, is a protein that in humans is encoded by the ESM1 gene. This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. ESM-1 has been described as a specific biomarker of tip cells during neoangiogenesis by independent teams. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF(vascular endothelial growth factor) or FGF-2(fibroblast growth factor 2).

Synonyms: Endothelial cell-specific molecule 1,ESM-1,Esm1,

Full Gene Name: Endothelial cell-specific molecule 1

Cellular Localisation: Secreted.
Gene ID 71690
UniProt Q9QYY7
Pathways Growth Factor Binding
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated
Protocol mouse ESM1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for ESM1 has been precoated onto 96-well plates. Standards(NSO, W20-R184) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ESM1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse ESM1 amount of sample captured in plate.
Assay Procedure

Aliquot 0.1 mL per well of the 1500pg/mL, 750pg/mL, 375pg/mL, 187.5pg/mL, 93.7pg/mL, 46.8pg/mL, 23.4pg/mL mouse ESM1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse ESM1 standard solution and each sample be measured in duplicate.

Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 56, Standard deviation: 1.85, CV(%): 3.3
  • Sample 2: n=16, Mean(pg/ml): 673, Standard deviation: 31.6, CV(%): 4.7
  • Sample 3: n=16, Mean(pg/ml): 1033, Standard deviation: 57, CV(%): 5.5,
  • Sample 1: n=24, Mean(pg/ml): 47, Standard deviation: 1.93, CV(%): 4.1
  • Sample 2: n=24, Mean(pg/ml): 660, Standard deviation: 34.98, CV(%): 5.3
  • Sample 3: n=24, Mean(pg/ml): 1135, Standard deviation: 72.64, CV(%): 6.4
Restrictions For Research Use only
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months
Supplier Images
ELISA image for Endothelial Cell-Specific Molecule 1 (ESM1) ELISA Kit (ABIN1889318) Mouse ESM1/Endocan PicoKine ELISA Kit standard curve
Background publications Lassalle, Molet, Janin, Heyden, Tavernier, Fiers, Devos, Tonnel: "ESM-1 is a novel human endothelial cell-specific molecule expressed in lung and regulated by cytokines." in: The Journal of biological chemistry, Vol. 271, Issue 34, pp. 20458-64, 1996 (PubMed).

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