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MME ELISA Kit

MME Reactivity: Human AA 52-750 Colorimetric Sandwich ELISA 125-8000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN1889325
  • Target See all MME ELISA Kits
    MME (Membrane Metallo-Endopeptidase (MME))
    Binding Specificity
    AA 52-750
    Reactivity
    • 4
    • 3
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    125-8000 pg/mL
    Minimum Detection Limit
    125 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CD10/Neprilysin
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: CHO
    Immunogen sequence: Y52-W750
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: CHO
    Immunogen sequence: Y52-W750
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Belongs to the peptidase M13 family.

    Plate
    Pre-coated
    Protocol
    human CD10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD10 has been precoated onto 96-well plates. Standards(CHO, Y52-W750) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD10 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL human CD10 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CD10 standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 1265, Standard deviation: 48.07, CV(%): 3.8
    • Sample 2: n=16, Mean(pg/ml): 3210, Standard deviation: 179.8, CV(%): 5.6
    • Sample 3: n=16, Mean(pg/ml): 6840, Standard deviation: 321.5, CV(%): 4.7,
    • Sample 1: n=24, Mean(pg/ml): 1526, Standard deviation: 83.93, CV(%): 5.5
    • Sample 2: n=24, Mean(pg/ml): 2761, Standard deviation: 201, CV(%): 7.3
    • Sample 3: n=24, Mean(pg/ml): 6397, Standard deviation: 369.6, CV(%): 6.2
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all MME ELISA Kits
    MME (Membrane Metallo-Endopeptidase (MME))
    Alternative Name
    MME (MME Products)
    Synonyms
    CALLA ELISA Kit, CD10 ELISA Kit, NEP ELISA Kit, SFE ELISA Kit, 6030454K05Rik ELISA Kit, C85356 ELISA Kit, Nep ELISA Kit, neprilysin ELISA Kit, membrane metalloendopeptidase ELISA Kit, membrane metallo endopeptidase ELISA Kit, membrane metallo-endopeptidase ELISA Kit, MME ELISA Kit, Mme ELISA Kit
    Target Type
    Chemical
    Background

    Protein Function: Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed:15283675, PubMed:8168535). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed:17101991). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed:15283675). Involved in the degradation of atrial natriuretic factor (ANF) (PubMed:2531377, PubMed:2972276). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573). .

    Background: CD10, also known as membrane metallo-endopeptidase, neutral endopeptidase(NEP), Neprilysin, or common acute lymphoblastic leukemia antigen(CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably theamyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease. This gene is localized to human chromosome 3 by study of somatic cell hybrids and regionalized the location to 3q21-q27 by in situ hybridization. By cDNA transfection analysis, CD10 is confirmed as a functional neutral endopeptidase of the type that has previously been called enkephalinase. CD10 has also been called atriopeptidase. Atriopeptidase specifically degrades atrial natriuretic factor. A specific enzyme inhibitor was developed and reported that it had effects similar to those of low-dose ANF infusion. These effects include diuresis, natriuresis, vasodilatation, and suppression of the renin-angiotensin-aldosterone system.

    Synonyms: Neprilysin,3.4.24.11 ,Atriopeptidase,Common acute lymphocytic leukemia antigen,CALLA,Enkephalinase,Neutral endopeptidase 24.11,NEP,Neutral endopeptidase,Skin fibroblast elastase,SFE,CD10,MME,EPN,

    Full Gene Name: Neprilysin

    Cellular Localisation: Cell membrane, Single-pass type II membrane protein.
    Gene ID
    4311
    UniProt
    P08473
    Pathways
    RTK Signaling, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Smooth Muscle Cell Migration
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