TGFB2 ELISA Kit

Catalog No. ABIN1889331

Product Details TGFB2 ELISA Kit

Target: TGFB2 (Transforming Growth Factor, beta 2 (TGFB2))

Binding Specificity: AA 303-414

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2-2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of activated Rat TGF-beta 2

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: A303-S414

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: A303-S414

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Tissue Specificity: Isoform TGF-beta2B expressed in the aorta, primary bronchus, uterus, heart, skeletal muscle, sciatic nerve and spinal cord but not in the intestine. .

Plate: Pre-coated

Protocol: rat TGF-beta 2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGF-beta 2 has been precoated onto 96-well plates. Standards(NSO, A303-S414) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGF-beta 2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat TGF-beta 2 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL rat TGF-beta 2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of activated rat cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat TGF-beta 2 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 304, Standard deviation: 16.42, CV(%): 5.4
• Sample 2: n=16, Mean(pg/ml): 614, Standard deviation: 31.93, CV(%): 5.2
• Sample 3: n=16, Mean(pg/ml): 1172, Standard deviation: 79.7, CV(%): 6.8,
• Sample 1: n=24, Mean(pg/ml): 377, Standard deviation: 23.75, CV(%): 6.3
• Sample 2: n=24, Mean(pg/ml): 536, Standard deviation: 39.13, CV(%): 7.3
• Sample 3: n=24, Mean(pg/ml): 1223, Standard deviation: 88.1, CV(%): 7.2

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for TGFB2 ELISA Kit (ABIN1889331)

Li, Liu, Shen, Wang, Chen, Xu, Wen: "MicroRNA-26a modulates transforming growth factor beta-1-induced proliferation in human fetal lung fibroblasts." in: Biochemical and biophysical research communications, Vol. 454, Issue 4, pp. 512-517, (2014) (PubMed).

Target Details for TGFB2

Target: TGFB2 (Transforming Growth Factor, beta 2 (TGFB2))

Alternative Name: TGFB2 (TGFB2 Products)

Synonyms: LDS4 ELISA Kit, TGF-beta2 ELISA Kit, tgf-beta2 ELISA Kit, tgfb2-A ELISA Kit, BB105277 ELISA Kit, Tgf-beta2 ELISA Kit, Tgfb-2 ELISA Kit, TGF-beta 2 ELISA Kit, Tgfbr2T ELISA Kit, TGFB2 ELISA Kit, TGFbeta2 ELISA Kit, MGF ELISA Kit, TGF-B2 ELISA Kit, transforming growth factor beta 2 ELISA Kit, transforming growth factor beta 2 L homeolog ELISA Kit, transforming growth factor, beta 2 ELISA Kit, transforming growth factor, beta receptor 2 ELISA Kit, TGFB2 ELISA Kit, tgfb2.L ELISA Kit, Tgfb2 ELISA Kit, Tgfbr2 ELISA Kit, tgfb2 ELISA Kit

Background:

Protein Function: TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth.

Background: Transforming growth factor-beta 2(TGF-beta 2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. This gene is mapped to 1q41. It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. TGF-beta 2 is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma(POAG). Studies have shown that TGF-beta 2 influences cultured trabecular meshwork cells, and it reduced outflow facility when perfused into cultured human anterior segments. In POAG, elevated expression of Gremlin by TM cells inhibited BMP4 antagonism of TGF-beta 2 and led to increased extracellular matrix deposition and elevated IOP.

Synonyms: Transforming growth factor beta-2,TGF-beta-2,Latency-associated peptide,LAP,Tgfb2,

Full Gene Name: Transforming growth factor beta-2

Cellular Localisation: Secreted.

Gene ID: 81809

UniProt: Q07257

Pathways: Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus