Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

CYR61 ELISA Kit

CYR61 Reactivity: Human AA 22-381 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine
Catalog No. ABIN1889372
  • Target See all CYR61 ELISA Kits
    CYR61 (Cysteine-Rich, Angiogenic Inducer, 61 (CYR61))
    Binding Specificity
    AA 22-381
    Reactivity
    • 6
    • 3
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    31.2-2000 pg/mL
    Minimum Detection Limit
    31.2 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CYR61/CCN1
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva, Urine, Milk
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: CHO
    Immunogen sequence: A22-D381
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: CHO
    Immunogen sequence: A22-D381
    Featured
    Discover our best selling CYR61 ELISA Kit
    Top Product
    Discover our top product CYR61 ELISA Kit
  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Belongs to the CCN family.

    Plate
    Pre-coated
    Protocol
    human CYR61 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CYR61 has been precoated onto 96-well plates. Standards(CHO, A22-D381) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CYR61 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CYR61 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CYR61 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva, urine or human milk to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CYR61 standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 628, Standard deviation: 19.5, CV(%): 3.1
    • Sample 2: n=16, Mean(pg/ml): 1145, Standard deviation: 36.6, CV(%): 3.2
    • Sample 3: n=16, Mean(pg/ml): 1574, Standard deviation: 53.5, CV(%): 3.4,
    • Sample 1: n=24, Mean(pg/ml): 570, Standard deviation: 27.93, CV(%): 4.9
    • Sample 2: n=24, Mean(pg/ml): 1097, Standard deviation: 58.1, CV(%): 5.3
    • Sample 3: n=24, Mean(pg/ml): 1563, Standard deviation: 96.9, CV(%): 6.2
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all CYR61 ELISA Kits
    CYR61 (Cysteine-Rich, Angiogenic Inducer, 61 (CYR61))
    Alternative Name
    CYR61 (CYR61 Products)
    Synonyms
    CCN1 ELISA Kit, GIG1 ELISA Kit, IGFBP10 ELISA Kit, ccn1 ELISA Kit, gig1 ELISA Kit, Xcyr61 ELISA Kit, igfbp10 ELISA Kit, MGC69281 ELISA Kit, MGC69539 ELISA Kit, cyr61-l2 ELISA Kit, zgc:158158 ELISA Kit, CYR61 ELISA Kit, AI325051 ELISA Kit, Igfbp10 ELISA Kit, cysteine rich angiogenic inducer 61 ELISA Kit, cysteine-rich, angiogenic inducer, 61 ELISA Kit, cysteine-rich, angiogenic inducer, 61 like 2 ELISA Kit, cysteine rich protein 61 ELISA Kit, cysteine rich angiogenic inducer 61 S homeolog ELISA Kit, CYR61 ELISA Kit, Cyr61 ELISA Kit, cyr61 ELISA Kit, cyr61l2 ELISA Kit, cyr61.S ELISA Kit
    Background

    Protein Function: Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up- regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. .

    Background: Cysteine-rich angiogenic inducer 61(CYR61) or CCN family member 1(CCN1), is a matricellular protein that in humans is encoded by the CYR61 gene. This gene is mapped to 1p22.3. CYR61 is capable of regulating a broad range of cellular activities, including cell adhesion, migration, proliferation, differentiation, apoptosis, and senescence through interaction with cell surface integrin receptors and heparan sulfate proteoglycans. During embryonic development, CYR61 is critical for cardiac septal morphogenesis, blood vessel formation in placenta, and vascular integrity. In adulthood CYR61 plays important roles in inflammation and tissue repair, and is associated with diseases related to chronic inflammation, including rheumatoid arthritis, atherosclerosis, diabetes-related nephropathy and retinopathy, and many different forms of cancers.

    Synonyms: Protein CYR61,CCN family member 1,Cysteine-rich angiogenic inducer 61,Insulin-like growth factor-binding protein 10,IBP-10,IGF-binding protein 10,IGFBP-10,Protein GIG1,CYR61,CCN1, GIG1, IGFBP10,

    Full Gene Name: Protein CYR61

    Cellular Localisation: Secreted.
    Gene ID
    3491
    UniProt
    O00622
    Pathways
    Positive Regulation of Endopeptidase Activity, Growth Factor Binding
You are here:
Support