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LUM ELISA Kit

LUM Reactivity: Rat AA 19-338 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Catalog No. ABIN1889413
  • Target See all LUM ELISA Kits
    LUM (Lumican (LUM))
    Binding Specificity
    AA 19-338
    Reactivity
    • 5
    • 3
    • 1
    • 1
    • 1
    Rat
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    156-10.000 pg/mL
    Minimum Detection Limit
    156 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat Lumican
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: Q19-N338
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: Q19-N338
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.

    Plate
    Pre-coated
    Protocol
    rat Lumican ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Lumican has been precoated onto 96-well plates. Standards(NSO, Q19-N338) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Lumican is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Lumican amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL rat Lumican standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat Lumican standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 1258, Standard deviation: 59.13, CV(%): 4.7
    • Sample 2: n=16, Mean(pg/ml): 3733, Standard deviation: 201.6, CV(%): 5.4
    • Sample 3: n=16, Mean(pg/ml): 6233, Standard deviation: 374, CV(%): 6,
    • Sample 1: n=24, Mean(pg/ml): 1476, Standard deviation: 87.1, CV(%): 5.9
    • Sample 2: n=24, Mean(pg/ml): 3946, Standard deviation: 248.6, CV(%): 6.3
    • Sample 3: n=24, Mean(pg/ml): 6852, Standard deviation: 500.2, CV(%): 7.3
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all LUM ELISA Kits
    LUM (Lumican (LUM))
    Alternative Name
    LUM (LUM Products)
    Synonyms
    MGC64527 ELISA Kit, zgc:86661 ELISA Kit, ldc ELISA Kit, slrr2d ELISA Kit, DKFZp468L1116 ELISA Kit, LDC ELISA Kit, SLRR2D ELISA Kit, Ldc ELISA Kit, lumican ELISA Kit, lumican L homeolog ELISA Kit, LUM ELISA Kit, lum.L ELISA Kit, lum ELISA Kit, CpipJ_CPIJ010209 ELISA Kit, Lum ELISA Kit
    Background

    Background: Lumican, also known as LUM, is a protein which in humans is encoded by the LUM gene. This gene is a member of the small interstitial proteoglycan gene(SIPG) family, and it is a keratan sulfate proteoglycan which presents large quantities in the corneal stroma and in interstitial collagenous matrices of the heart, aorta, skeletal muscle, skin, and intervertebral discs. Lumican is mapped to 12q21.33, it interacts with collagen and limits growth of fibrils in diameter. In the cornea, Lumican not only interacts with collagen molecules to limit fibril growth, but also plays a critical role in the regular spacing of fibrils and acquisition of corneal transparency by virtue of its keratan sulfate-containing glycosaminoglycan side chains LDC.

    Synonyms: Lumican,Keratan sulfate proteoglycan lumican,KSPG lumican,Lum,Lcn, Ldc,

    Full Gene Name: Lumican

    Cellular Localisation: Secreted, extracellular space, extracellular matrix.
    Gene ID
    81682
    UniProt
    P51886
    Pathways
    Glycosaminoglycan Metabolic Process
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