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Sialoadhesin/CD169 ELISA Kit

SIGLEC1 Reactivity: Mouse AA 20-1639 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN1889445
  • Target See all Sialoadhesin/CD169 (SIGLEC1) ELISA Kits
    Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))
    Binding Specificity
    AA 20-1639
    Reactivity
    • 5
    • 2
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    156-10.000 pg/mL
    Minimum Detection Limit
    156 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD169/SIGLEC-1
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: T20-R1639
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: T20-R1639
    Top Product
    Discover our top product SIGLEC1 ELISA Kit
  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.

    Tissue Specificity: Detected in lymph node in the subcapsular sinus, interfollicular regions, and T and B-cell boundary (at protein level). Expressed by macrophages in various tissues. Highest expression in spleen and lymph node with lower amounts in lung, liver, bone marrow, heart and skin. No expression in thymus, kidney, brain or small intestine. .

    Plate
    Pre-coated
    Protocol
    mouse CD169 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD169 has been precoated onto 96-well plates. Standards(NSO, T20-R1639) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD169 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD169 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse CD169 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CD169 standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(ng/ml): 1.64, Standard deviation: 0.06, CV(%): 3.7
    • Sample 2: n=16, Mean(ng/ml): 4.85, Standard deviation: 0.204, CV(%): 4.2
    • Sample 3: n=16, Mean(ng/ml): 6.88, Standard deviation: 0.316, CV(%): 4.6,
    • Sample 1: n=24, Mean(ng/ml): 2.54, Standard deviation: 0.119, CV(%): 4.7
    • Sample 2: n=24, Mean(ng/ml): 5.31, Standard deviation: 0.27, CV(%): 5.1
    • Sample 3: n=24, Mean(ng/ml): 7.05, Standard deviation: 0.437, CV(%): 6.2
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all Sialoadhesin/CD169 (SIGLEC1) ELISA Kits
    Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))
    Alternative Name
    SIGLEC1 (SIGLEC1 Products)
    Synonyms
    Cd169 ELISA Kit, Siglec-1 ELISA Kit, Sn ELISA Kit, CD169 ELISA Kit, SIGLEC-1 ELISA Kit, SN ELISA Kit, dJ1009E24.1 ELISA Kit, SA ELISA Kit, SIGLEC1 ELISA Kit, p210 ELISA Kit, pSn ELISA Kit, sialic acid binding Ig like lectin 1 ELISA Kit, sialic acid binding Ig-like lectin 1, sialoadhesin ELISA Kit, SIGLEC1 ELISA Kit, Siglec1 ELISA Kit
    Background

    Protein Function: Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells (By similarity). Preferentially binds to alpha- 2,3-linked sialic acid. Binds to SPN/CD43 on T-cells. May play a role in hematopoiesis. May act as a counter-receptor for CLEC10A in lymph node. .

    Background: SIGLEC-1, also known as Sialoadhesin or CD169, is a cell adhesion molecule found on the surface of certain cells of the immune system called macrophages. This gene is mapped to 20p13. It belongs to the immunoglobulin superfamily(IgSF). Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. The localization and expression of SIGLEC-1 in humans has altered coincident with the evolutionary loss of Neu5Gc. SIGLEC-1-positive macrophages have a dual physiologic function. They act as innate 'flypaper' by preventing the systemic spread of lymph-borne pathogens and as critical gatekeepers at the lymph-tissue interface that facilitate the recognition of particulate antigens by B cells and initiate humoral immune responses.

    Synonyms: Sialoadhesin,Sheep erythrocyte receptor,SER,Sialic acid-binding Ig-like lectin 1,Siglec-1,CD169,Siglec1,Sa, Sn,

    Full Gene Name: Sialoadhesin

    Cellular Localisation: Isoform 1: Cell membrane, Single-pass type I membrane protein.
    Gene ID
    20612
    UniProt
    Q62230
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