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TNFSF18 ELISA Kit

TNFSF18 Reactivity: Mouse AA 47-173 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN1889448
  • Target See all TNFSF18 ELISA Kits
    TNFSF18 (Tumor Necrosis Factor (Ligand) Superfamily, Member 18 (TNFSF18))
    Binding Specificity
    AA 47-173
    Reactivity
    • 3
    • 3
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    31.2-2000 pg/mL
    Minimum Detection Limit
    31.2 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse TNFSF18/GITRL
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: T47-S173
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: T47-S173
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Tissue Specificity: Detected in immature and mature dendritic cells and in macrophages (at protein level). Detected in spleen, lung, heart, thymus, monocytes, macrophages, B-cells and dendritic cells. .

    Plate
    Pre-coated
    Protocol
    mouse TNFSF18 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFSF18 has been precoated onto 96-well plates. Standards(NSO, T47-S173) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFSF18 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFSF18 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse TNFSF18 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TNFSF18 standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 183, Standard deviation: 7.7, CV(%): 4.2
    • Sample 2: n=16, Mean(pg/ml): 764, Standard deviation: 35.2, CV(%): 4.6
    • Sample 3: n=16, Mean(pg/ml): 1360, Standard deviation: 51.7, CV(%): 3.8,
    • Sample 1: n=24, Mean(pg/ml): 212, Standard deviation: 12.1, CV(%): 5.7
    • Sample 2: n=24, Mean(pg/ml): 862, Standard deviation: 53.4, CV(%): 6.2
    • Sample 3: n=24, Mean(pg/ml): 1505, Standard deviation: 72.2, CV(%): 4.8
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all TNFSF18 ELISA Kits
    TNFSF18 (Tumor Necrosis Factor (Ligand) Superfamily, Member 18 (TNFSF18))
    Alternative Name
    TNFSF18 (TNFSF18 Products)
    Synonyms
    Gitrl ELISA Kit, AITRL ELISA Kit, GITRL ELISA Kit, TL6 ELISA Kit, hGITRL ELISA Kit, TNLG2A ELISA Kit, tumor necrosis factor (ligand) superfamily, member 18 ELISA Kit, TNF superfamily member 18 ELISA Kit, Tnfsf18 ELISA Kit, TNFSF18 ELISA Kit
    Background

    Protein Function: Cytokine that binds to TNFRSF18/AITR/GITR (PubMed:14521928, PubMed:14647196). Regulates T-cell responses (PubMed:14647196). Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation (PubMed:14608036, PubMed:15128759). Important for interactions between activated T-lymphocytes and endothelial cells. Mediates activation of NF-kappa-B (PubMed:14521928, PubMed:14647196, PubMed:18178614). Triggers increased phosphorylation of STAT1 and up-regulates expression of VCAM1 and ICAM1 (By similarity). Promotes leukocyte adhesion to endothelial cells (PubMed:23892569). Regulates migration of monocytes from the splenic reservoir to sites of inflammation (PubMed:24107315). .

    Background: Tumor necrosis factor ligand superfamily member 18, also known as AITRL or GITRL, is a protein that in humans is encoded by the TNFSF18 gene. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor(TNF) ligand family. It is mapped to 1q25.1. TNFSF18 is a ligand for receptor TNFRSF18/AITR/GITR. It has been shown to modulate T lymphocyte survival in peripheral tissues. This cytokine is also found to be expressed in endothelial cells, and is thought to be important for interaction between T lymphocytes and endothelial cells. TNFSF18-dependent modulation of tryptophan catabolism may represent an important mechanism of action of glucocorticoids, both physiologically and therapeutically.

    Synonyms: Tumor necrosis factor ligand superfamily member 18,GITR ligand,GITRL,Glucocorticoid-induced TNF-related ligand,Tnfsf18,Gitrl,

    Full Gene Name: Tumor necrosis factor ligand superfamily member 18

    Cellular Localisation: Cell membrane, Single-pass type II membrane protein.
    Gene ID
    240873
    UniProt
    Q7TS55
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