MK2-Trap

Details for Product No. ABIN1889472, Supplier: Log in to see
Antigen
  • mapkapk2
  • wu:fb30e10
  • wu:fc56c10
  • wu:fi03a01
  • MGC78852
  • MAPKAPK2
  • AA960234
  • MAPKAP-K2
  • MK-2
  • MK2
  • Rps6kc1
  • mitogen-activated protein kinase-activated protein kinase 2a
  • mitogen-activated protein kinase-activated protein kinase 2
  • MAP kinase-activated protein kinase 2
  • mapkapk2a
  • mapkapk2
  • MAPKAPK2
  • Mapkapk2
Alternatives
Human MAPKAP Kinase 2 ELISA Kit
Reactivity
Hamster, Human, Mouse (Murine)
11
11
9
2
Host
Camelidae
Antibody Type
Recombinant Antibody
Application
Affinity Measurement (AM), Chromatin Immunoprecipitation (ChIP), Enzyme Activity Assay (EAA), Immunoprecipitation (IP), Mass Spectrometry (MS), Protein Complex Immunoprecipitation (Co-IP), Pull-Down Assay (Pull-Down), Purification (Purif)
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Purpose The MK2-Trap is a high quality MK2-binding protein for biochemical analysis of MK2 and interacting partners.
Sample Type Cell Extracts
Specificity Posttranslational Modifications: MK2-Trap recognizes unphosphorylated MK2 and Phospho-MK2 (Thr222). Specificity on Phospho-MK2 (Thr334) was not tested.
Characteristics MK2-Trap is excellent for fast and efficient one-step isolation of MK2 and its interacting factors from cellular extract. Isolated MK2 protein may be used further for immunoblot analysis, mass spectrometry, and kinase assays.
MK2-Trap utilizes small recombinant alpaca antibody fragments covalently coupled to the surface of agarose beads.
Purification Purifed protein
Components MK2-Trap uncoupled
Material not included Lysis buffer (CoIP), 10x RIPA buffer, Dilution buffer, Wash buffer, Elution buffer
Alternative Name MK2 (MAPKAPK2 ELISA Kit Abstract)
Background Mitogen-activated protein kinase-activated protein kinase 2 (MAPKAPK2 / MK2) belongs to the family of serine/threonine kinases. In response to cellular stress it is phosphorylated and activated by MAP kinase p38.
Comment

Use this MK2-Trap to couple it to your own immobilizing matrix.

Assay Time 1.5 h
Protocol
  • Robust and versatile tool for biochemical analyses of MK2 proteins
  • Short incubation times (5 - 30 min)
  • Quantitative isolation of fusion proteins and transiently bound factors from cell extracts or organelles
  • Low unspecific binding
  • No contaminating heavy and light chains of conventional antibodies
  • Applicable in Chromatin Immunoprecipitation (ChIP)
Sample Collection Harvest cells:
For one immunoprecipitation reaction the use of 10^6 - 10^7 mammalian cells (approx. one 10 cm dish) is recommended. To harvest adherent cells, aspirate growth medium, add 1 ml ice-cold PBS to cells and scrape cells from dish. Transfer cells to a pre-cooled tube, spin at 500 g for 3 min at +4°C and discard supernatant. Wash cell pellet twice with ice-cold PBS, gently resuspending the cells.

Lyse cells
  • 1. Resuspend cell pellet in 200 µL ice-cold lysis buffer by pipetting or using a syringe.
    note: Supplement lysis buffer with protease inhibitors and 1 mM PMSF (not included). optional for nuclear/chromatin proteins: Use RIPA buffer supplemented with 1 mg/mL DNase, 2.5 mM MgCl2, protease inhibitors and 1 mM PMSF (not included).
  • 2. Place the tube on ice for 30 min with extensively pipetting every 10 min.
  • 3. Centrifuge cell lysate at 20.000x g for 10 min at +4°C. Transfer lysate to a pre-cooled tube. Add 300 µl dilution buffer to lysate. Discard pellet.
    note: At this point cell lysate may be put at -80°C for long-term storage. optional: Add 1 mM PMSF and protease inhibitors (not included) to dilution buffer

We recommend that during incubation with the beads the final concentration of detergents does not exceed 0.2% to avoid unspecific binding to the matrix. If required, use more dilution buffer to dilute the supernatant accordingly.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer 1 x PBS, 0.01 % Sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Expiry Date 12 months
Supplier Images
Immunoprecipitation (IP) image for MK2-Trap (ABIN1889472) Pulldown of MK2 using the MK2-Trap: Immunoprecipitations (IP) of MK2 from a protein e...
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