Mitochondrial Permeability Transition Pore Assay Kit

Details for Product No. ABIN2015837, Supplier: Log in to see
Reactivity
Mammalian
Application
Activity Assay (AcA), Detection (D), Flow Cytometry (FACS)
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Sample Type Biological Fluids, Adherent Cell Culture
Detection Method Fluorometric
Characteristics Simple procedure
Fast, convenient and sensitive
The assay provides a direct method of measuring mitochondrial permeability transition pore activation as an early marker of apoptosis
Components MPTP Staining Dye
CoCl2
Ionomycin
MPTP Wash Buffer
Background Mitochondria are the power centrals of the cell and play an essential role in energy production. Damage to mitochondria activates signaling pathways that induce apoptosis. The mitochondrial permeability transition pore (MPT pore or MPTP) is a nonspecific channel formed by components of the inner and outer mitochondrial membranes, and appears to be involved in the release of mitochondrial components during cell death. In healthy cells, MPTP's flicker between open and closed states but during cell death MPTP's dramatically alter the permeability of the mitochondria. Cytochrome c release and loss of mitochondrial membrane potential are subsequent to continuous pore activation.
Application Notes Measure mitochondrial permeability transition pore activity in cells Assessment of physiological mediators and antibodies that affect cell viability/cytotoxicity
Comment

Further details regarding sample type: Cells (adherent and suspension)

Restrictions For Research Use only
Storage -20 °C
Expiry Date 12 months
Supplier Images
Flow Cytometry (FACS) image for Mitochondrial Permeability Transition Pore Assay Kit (ABIN2015837) Mitochondrial Permeability Transition Pore Assay Kit
Product cited in: Li, Zhao, Zhou, Zhang, Qin: "Exendin-4 promotes endothelial barrier enhancement via PKA- and Epac1-dependent Rac1 activation." in: American journal of physiology. Cell physiology, Vol. 308, Issue 2, pp. C164-75, 2015 (PubMed).