ADCY10
Reactivity: Human
Soluble
Colorimetric
78-5000 pg/mL
Plate
Pre-coated
Protocol
Adenylate Cyclase 10, Soluble (ADCY10) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenylate Cyclase 10, Soluble (ADCY10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenylate Cyclase 10, Soluble (ADCY10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenylate Cyclase 10, Soluble (ADCY10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Adenylate Cyclase 10, Soluble (ADCY10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.