BHMT ELISA Kit

Catalog No. ABIN2043009

Product Details BHMT ELISA Kit

Target: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Minimum Detection Limit: 0.31 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.124 ng/mL

Application Details

Plate: Pre-coated

Protocol: Betaine Homocysteine Methyltransferase (BHMT) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Betaine Homocysteine Methyltransferase (BHMT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Betaine Homocysteine Methyltransferase (BHMT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Betaine Homocysteine Methyltransferase (BHMT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Betaine Homocysteine Methyltransferase (BHMT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for BHMT

Target: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Alternative Name: Betaine Homocysteine Methyltransferase (BHMT) (BHMT Products)

Synonyms: BHMT1 ELISA Kit, hm:zehn2153 ELISA Kit, wu:fb53h01 ELISA Kit, wu:fb63c08 ELISA Kit, wu:fj64d01 ELISA Kit, zgc:123027 ELISA Kit, betaine--homocysteine S-methyltransferase ELISA Kit, betaine--homocysteine S-methyltransferase L homeolog ELISA Kit, betaine-homocysteine methyltransferase ELISA Kit, betaine-homocysteine S-methyltransferase ELISA Kit, BHMT ELISA Kit, bhmt.L ELISA Kit, Bhmt ELISA Kit, bhmt ELISA Kit

Pathways: Methionine Biosynthetic Process