CACNA1B ELISA Kit

Catalog No. ABIN2043045

Product Details CACNA1B ELISA Kit

Target: CACNA1B (Calcium Channel, Voltage-Dependent, N Type, alpha 1B Subunit (CACNA1B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Minimum Detection Limit: 0.31 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.129 ng/mL

Application Details

Plate: Pre-coated

Protocol: Calcium Channel, Voltage Dependent, N-Type, Alpha 1B Subunit (CACNa1B) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium Channel, Voltage Dependent, N-Type, Alpha 1B Subunit (CACNa1B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium Channel, Voltage Dependent, N-Type, Alpha 1B Subunit (CACNa1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium Channel, Voltage Dependent, N-Type, Alpha 1B Subunit (CACNa1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Calcium Channel, Voltage Dependent, N-Type, Alpha 1B Subunit (CACNa1B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CACNA1B

Target: CACNA1B (Calcium Channel, Voltage-Dependent, N Type, alpha 1B Subunit (CACNA1B))

Alternative Name: Calcium Channel, Voltage Dependent, N-Type, alpha 1B Subunit (CACNa1B) (CACNA1B Products)

Synonyms: BIII ELISA Kit, CACNL1A5 ELISA Kit, CACNN ELISA Kit, Cav2.2 ELISA Kit, AW050276 ELISA Kit, AW060892 ELISA Kit, AW822256 ELISA Kit, Cchn1a ELISA Kit, alpha(1B) ELISA Kit, calcium voltage-gated channel subunit alpha1 B ELISA Kit, calcium channel, voltage-dependent, N type, alpha 1B subunit ELISA Kit, CACNA1B ELISA Kit, Cacna1b ELISA Kit