Choline Acetyltransferase ELISA Kit
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- Target See all Choline Acetyltransferase (CHAT) ELISA Kits
- Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.15-10 ng/mL
- Minimum Detection Limit
- 0.15 ng/mL
- Application
- ELISA
- Brand
- BioAssay™
- Sensitivity
- < 0.056 ng/mL
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Choline O-Acetyltransferase (CHAT) ELISA Kit
CHAT Reactivity: Rat Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Choline O-Acetyltransferase (CHAT) ELISA KitCHAT Reactivity: Mouse Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Choline O-Acetyltransferase (CHAT) ELISA KitCHAT Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Choline O-Acetyltransferase (CHAT) ELISA KitCHAT Reactivity: Mouse Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
Choline O-Acetyltransferase (CHAT) ELISA KitCHAT Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Choline O-Acetyltransferase (CHAT) ELISA KitCHAT Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- Plate
- Pre-coated
- Protocol
- Choline Acetyltransferase (ChAT) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Choline Acetyltransferase (ChAT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Choline Acetyltransferase (ChAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Choline Acetyltransferase (ChAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Choline Acetyltransferase (ChAT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C/-20 °C
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- Target See all Choline Acetyltransferase (CHAT) ELISA Kits
- Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))
- Alternative Name
- Choline Acetyltransferase (ChAT) (CHAT Products)
- Synonyms
- CHOACTASE ELISA Kit, CMS1A ELISA Kit, CMS1A2 ELISA Kit, ChoAcTase ELISA Kit, B230380D24Rik ELISA Kit, choline O-acetyltransferase ELISA Kit, choline O-acetyltransferase a ELISA Kit, choline acetyltransferase ELISA Kit, carnitine O-acetyltransferase ELISA Kit, CHAT ELISA Kit, chata ELISA Kit, Chat ELISA Kit, PGTG_00551 ELISA Kit, LOC100533322 ELISA Kit
- Pathways
- Skeletal Muscle Fiber Development
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