AKR1C2 ELISA Kit

Catalog No. ABIN2043343

Product Details AKR1C2 ELISA Kit

Target: AKR1C2 (Aldo-keto Reductase Family 1, Member C2 (AKR1C2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.061 ng/mL

Application Details

Plate: Pre-coated

Protocol: Dihydrodiol Dehydrogenase 2 (DDH2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dihydrodiol Dehydrogenase 2 (DDH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dihydrodiol Dehydrogenase 2 (DDH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dihydrodiol Dehydrogenase 2 (DDH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Dihydrodiol Dehydrogenase 2 (DDH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for AKR1C2

Target: AKR1C2 (Aldo-keto Reductase Family 1, Member C2 (AKR1C2))

Alternative Name: Dihydrodiol Dehydrogenase 2 (DDH2) (AKR1C2 Products)

Synonyms: AKR1C-pseudo ELISA Kit, BABP ELISA Kit, DD ELISA Kit, DD2 ELISA Kit, DDH2 ELISA Kit, HAKRD ELISA Kit, HBAB ELISA Kit, MCDR2 ELISA Kit, SRXY8 ELISA Kit, Akr1c21 ELISA Kit, aldo-keto reductase family 1 member C2 ELISA Kit, aldo-keto reductase family 1, member C2 ELISA Kit, AKR1C2 ELISA Kit, Akr1c2 ELISA Kit

Pathways: Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process