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APOB ELISA Kit

APOB Reactivity: Mouse Colorimetric Sandwich ELISA 6.25-400 ng/mL

Catalog No. ABIN2043651

Datasheet as PDF

Target See all APOB ELISA Kits

APOB (Apolipoprotein B (APOB))
Reactivity
8

8

4

4

3

2

2

2

2

2

2

1

1

1

1
Mouse

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

6.25-400 ng/mL

Minimum Detection Limit

6.25 ng/mL

Application

ELISA

Brand

BioAssay™

Sensitivity

< 2.04 ng/mL

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Plate

Pre-coated

Protocol

High Sensitivity Apolipoprotein B (APOB) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein B (APOB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein B (APOB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein B (APOB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Apolipoprotein B (APOB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions

For Research Use only
Storage

4 °C/-20 °C
Target See all APOB ELISA Kits

APOB (Apolipoprotein B (APOB))

Abstract

APOB Products

Synonyms

FLDB ELISA Kit, LDLCQ4 ELISA Kit, Ac1-060 ELISA Kit, Apo B-100 ELISA Kit, ApoB-100 ELISA Kit, ApoB-48 ELISA Kit, AI315052 ELISA Kit, apob-100 ELISA Kit, apob-48 ELISA Kit, APOB-100 ELISA Kit, ApoB(100) ELISA Kit, APOB ELISA Kit, apolipoprotein B ELISA Kit, APOB ELISA Kit, Apob ELISA Kit

Pathways

Lipid Metabolism