NFAT1 ELISA Kit

Catalog No. ABIN2044119

Product Details NFAT1 ELISA Kit

Target: NFAT1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.62-1000 pg/mL

Minimum Detection Limit: 15.62 pg/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 5.9 pg/mL

Application Details

Plate: Pre-coated

Protocol: Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for NFAT1

Target: NFAT1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1))

Alternative Name: Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2) (NFAT1 Products)

Synonyms: NFATC2 ELISA Kit, AI607462 ELISA Kit, NF-ATc2 ELISA Kit, NFAT1 ELISA Kit, NFAT1-D ELISA Kit, Nfatp ELISA Kit, NFATP ELISA Kit, nuclear factor of activated T-cells 2 ELISA Kit, nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2 ELISA Kit, NFATC2 ELISA Kit, nfatc2 ELISA Kit, Nfatc2 ELISA Kit

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, VEGF Signaling, BCR Signaling