RNASE2 ELISA Kit

Catalog No. ABIN2044445

Product Details RNASE2 ELISA Kit

Target: RNASE2 (Ribonuclease, RNase A Family, 2 (Liver, Eosinophil-Derived Neurotoxin) (RNASE2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Minimum Detection Limit: 0.31 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.117 ng/mL

Application Details

Plate: Pre-coated

Protocol: Ribonuclease A2 (RNASE2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonuclease A2 (RNASE2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonuclease A2 (RNASE2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonuclease A2 (RNASE2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ribonuclease A2 (RNASE2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for RNASE2

Target: RNASE2 (Ribonuclease, RNase A Family, 2 (Liver, Eosinophil-Derived Neurotoxin) (RNASE2))

Alternative Name: Ribonuclease A2 (RNASE2) (RNASE2 Products)

Synonyms: EDN ELISA Kit, RNS2 ELISA Kit, T5I7.8 ELISA Kit, T5I7_8 ELISA Kit, ribonuclease 2 ELISA Kit, RNase 2 ELISA Kit, ribonuclease A family member 2 ELISA Kit, ribonuclease 2 ELISA Kit, RNASE2 ELISA Kit, RNS2 ELISA Kit