UGCG ELISA Kit (UDP-Glucose Ceramide Glucosyltransferase)

Details for Product UGCG ELISA Kit No. ABIN2044747, Supplier: Log in to see
Antigen
  • gcs
  • glct1
  • ugcg
  • ugcgb
  • xlcgt
  • cb539
  • sb:cb539
  • zgc:112506
  • GCS
  • GLCT1
  • AU043821
  • C80537
  • Epcs21
  • GlcT-1
  • Ugcgl
  • UDP-glucose ceramide glucosyltransferase
  • UGCG
  • ugcg-b
  • ugcg
  • Ugcg
Reactivity
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
9
6
3
1
1
1
1
1
Method Type
Sandwich ELISA
Detection Range
1.56-100 ng/mL
Minimum Detection Limit
1.56 ng/mL
Application
ELISA
Options
Supplier
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Supplier Product No.
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Brand BioAssay™
Detection Method Colorimetric
Sensitivity < 0.65 ng/mL
Alternative Name UDP Glucose Ceramide Glucosyltransferase (UGCG) (UGCG ELISA Kit Abstract)
Plate Pre-coated
Protocol UDP Glucose Ceramide Glucosyltransferase (UGCG) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to UDP Glucose Ceramide Glucosyltransferase (UGCG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to UDP Glucose Ceramide Glucosyltransferase (UGCG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain UDP Glucose Ceramide Glucosyltransferase (UGCG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of UDP Glucose Ceramide Glucosyltransferase (UGCG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Restrictions For Research Use only
Storage 4 °C/-20 °C
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