WNT1 ELISA Kit

Catalog No. ABIN2044805

Product Details WNT1 ELISA Kit

Target: WNT1 (Wingless-Type MMTV Integration Site Family, Member 1 (WNT1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.053 ng/mL

Application Details

Plate: Pre-coated

Protocol: Wingless Type MMTV Integration Site Family, Member 1 (WNT1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 1 (WNT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 1 (WNT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wingless Type MMTV Integration Site Family, Member 1 (WNT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Wingless Type MMTV Integration Site Family, Member 1 (WNT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for WNT1

Target: WNT1 (Wingless-Type MMTV Integration Site Family, Member 1 (WNT1))

Alternative Name: Wingless Type MMTV Integration Site Family, Member 1 (WNT1) (WNT1 Products)

Synonyms: Xint-1 ELISA Kit, Xwnt1 ELISA Kit, int-1 ELISA Kit, int1 ELISA Kit, wnt-1 ELISA Kit, wnt1-a ELISA Kit, Int-1 ELISA Kit, Wnt-1 ELISA Kit, sw ELISA Kit, swaying ELISA Kit, BMND16 ELISA Kit, INT1 ELISA Kit, OI15 ELISA Kit, Int1 ELISA Kit, WNT-1 ELISA Kit, sb:eu647 ELISA Kit, zgc:194464 ELISA Kit, zgc:194478 ELISA Kit, WNT1 ELISA Kit, Wnt family member 1 ELISA Kit, Wnt family member 1 L homeolog ELISA Kit, wingless-type MMTV integration site family, member 1 ELISA Kit, WNT1 ELISA Kit, wnt1.L ELISA Kit, Wnt1 ELISA Kit, wnt1 ELISA Kit

Pathways: WNT Signaling, Dopaminergic Neurogenesis