SMAD, Mothers Against DPP Homolog 1 (SMAD1) ELISA Kit

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Antigen
  • MLP
  • Xmad1
  • Xsmad1
  • bsp1
  • jv4-1
  • jv41
  • madh1
  • madr1
  • smad-1
  • MADH1
  • fb39h09
  • wu:fb39h09
  • wu:fb97e04
  • 2/23
  • CG12399
  • Dmel\\CG12399
  • E(zen)2
  • En(vvl)
  • MAD
  • Mat
  • P-Mad
  • P-mad
  • PMad
  • Smad
  • Smad1
  • apg
  • c28
  • dMAD
  • dMad
  • l(2)K00237
  • l(2)k00237
  • mad
  • p-Mad
  • pMAD
  • pMad
  • pSmad
  • BSP-1
  • BSP1
  • JV4-1
  • JV41
  • MADR1
  • AI528653
  • Madh1
  • Madr1
  • Mlp1
  • MusMLP
  • smad1
  • xmad
  • SMAD family member 1
  • MAD homolog 1 (Drosophila)
  • Mothers against dpp
  • smad1
  • SMAD1
  • Mad
  • Smad1
  • smad1-a
Alternatives
Human SMAD, Mothers Against DPP Homolog 1 ELISA Kit
Epitope
pSer465
Reactivity
Human, Mouse (Murine), Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
25
20
16
4
3
2
2
1
1
1
1
Method Type
DNA-Binding ELISA
Application
ELISA
Options
Supplier
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Supplier Product No.
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Analytical Method Quantitative
Detection Method Colorimetric
Specificity The Smad1 (Phospho-Ser465) DNA-Binding ELISA detects endogenous levels of Smad1 only when phosphorylated at Ser465.
Characteristics Assay Type: DNA-Binding
Alternative Name Smad1 (SMAD1 ELISA Kit Abstract)
Background Synonyms: BSP-1, BSP1, Dwarfin-A, Dwf-A, JV4-1, MADH1, MADR1, Mad-related protein 1, Mad1, Mothers against DPP homolog 1, Mothers against decapentaplegic homolog 1, Mothers-against-DPP-related-1, SMAD 1, Smad 1, Transforming growth factor- beta signaling protein-1
Gene Symbol: SMAD1
Gene ID 4086
UniProt Q15797
Research Area Cancer, Growth Factors, Signaling, Transcription Factors
Pathways Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
Plate Pre-coated
Assay Procedure
  • Remove the coating solution and wash the plate three times by filling the wells with 100 μL PBS-0.05 % Tween20. The solutions or washes are removed by flicking the plate over a sink. The remaining drops are removed by patting the plate on a paper towel.
  • Block the remaining protein-binding sites in the coated wells by adding 100 μL blocking buffer, 3 % skim milk in PBS per well. Incubate for 1 hour at RT with gentle shaking.
  • Wash the plate three times with 100 μL PBS-0.05 % Tween 20.
  • Add 50 μL of diluted antibody to each well. Incubate the plate at 37 °C for an hour with gentle shaking.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween 20.
  • Add 50 μL of conjugated secondary antibody, diluted at the optimal concentration (according to the manufacturer) in blocking buffer immediately before use. Incubate at 37 °C for an hour.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween20.
  • Prepare the substrate solution by mixing acetic acid, TMB and 0.03 % H2O2 with the volume ratio of 4:1:5.
  • Dispense 50 μL of the substrate solution per well with a multichannel pipe. Incubate the plate at 37 °C in dark for 15-30 mins.
  • After sufficient color development, add 100 μL of stop solution to the wells (if necessary).
  • Read the absorbance (optical density at 450nm) of each well with a plate reader.
Restrictions For Research Use only
Handling Advice Avoid multiple freeze-thaw cycles
Storage 4 °C
Storage Comment Store at 4 °C for frequent use, at -20°C for infrequent use.
Expiry Date 6 months
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