CREB1 ELISA Kit (CAMP Responsive Element Binding Protein 1)

Details for Product CREB1 ELISA Kit No. ABIN2113333, Supplier: Log in to see
Antigen
  • creb
  • CREB1
  • Creb1
  • CREB
  • 2310001E10Rik
  • 3526402H21Rik
  • AV083133
  • Creb
  • Creb-1
  • creb1
  • zgc:55598
  • cAMP responsive element binding protein 1
  • cAMP responsive element binding protein 1a
  • cyclic AMP response element binding protein
  • CREB1
  • creb1
  • Creb1
  • LOC100351999
  • creb1a
  • CREB
Alternatives
Human CREB1 ELISA Kit
Epitope
pSer121
Reactivity
Human, Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
25
18
17
1
Method Type
DNA-Binding ELISA
Application
ELISA
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Analytical Method Quantitative
Detection Method Colorimetric
Specificity The CREB (Phospho-Ser121) DNA-Binding ELISA Kit detects endogenous levels of CREB only when phosphorylated at Ser121.
Characteristics Assay Type: DNA-Binding
Alternative Name CREB (CREB1 ELISA Kit Abstract)
Background Synonyms: cAMP responsive element binding protein 1, cAMP-response element binding protein, CREB-1, CREB1
Gene Symbol: CREB1
Gene ID 1385
UniProt P16220
Research Area Immunology, Innate Immunity, Transcription Factors
Pathways TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation
Plate Pre-coated
Assay Procedure
  • Remove the coating solution and wash the plate three times by filling the wells with 100 μL PBS-0.05 % Tween20. The solutions or washes are removed by flicking the plate over a sink. The remaining drops are removed by patting the plate on a paper towel.
  • Block the remaining protein-binding sites in the coated wells by adding 100 μL blocking buffer, 3 % skim milk in PBS per well. Incubate for 1 hour at RT with gentle shaking.
  • Wash the plate three times with 100 μL PBS-0.05 % Tween 20.
  • Add 50 μL of diluted antibody to each well. Incubate the plate at 37 °C for an hour with gentle shaking.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween 20.
  • Add 50 μL of conjugated secondary antibody, diluted at the optimal concentration (according to the manufacturer) in blocking buffer immediately before use. Incubate at 37 °C for an hour.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween20.
  • Prepare the substrate solution by mixing acetic acid, TMB and 0.03 % H2O2 with the volume ratio of 4:1:5.
  • Dispense 50 μL of the substrate solution per well with a multichannel pipe. Incubate the plate at 37 °C in dark for 15-30 mins.
  • After sufficient color development, add 100 μL of stop solution to the wells (if necessary).
  • Read the absorbance (optical density at 450nm) of each well with a plate reader.
Restrictions For Research Use only
Handling Advice Avoid multiple freeze-thaw cycles
Storage 4 °C
Storage Comment Store at 4 °C for frequent use, at -20°C for infrequent use.
Expiry Date 6 months
Did you look for something else?