GATA4 ELISA Kit (GATA Binding Protein 4)

Details for Product GATA4 ELISA Kit No. ABIN2113677, Supplier: Log in to see
Antigen
  • gata-4
  • xgata4
  • xGATA-4
  • MGC81427
  • GATA4
  • GATA-4
  • Gata4
  • gata4
  • ASD2
  • VSD1
  • Gata-4
  • gata
  • GATA binding protein 4
  • glutamyl-tRNA(Gln) amidotransferase subunit A
  • GATA-4 zinc-finger transcription factor
  • gata4 transcription factor
  • GATA4
  • GATA-binding protein 4
  • LOC100230031
  • gata4
  • GATA4
  • gatA4
  • GATA-4
  • LOC100534420
  • Gata4
  • gata4-b
Alternatives
Human GATA4 ELISA Kit
Epitope
pSer262
Reactivity
Human, Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
18
14
12
3
2
2
2
2
2
1
1
Method Type
DNA-Binding ELISA
Application
ELISA
Options
Supplier
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Supplier Product No.
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Analytical Method Quantitative
Detection Method Colorimetric
Specificity The GATA4 (Phospho-Ser262) DNA-Binding ELISA Kit detects endogenous levels of GATA4 only when phosphorylated at Ser262.
Characteristics Assay Type: DNA-Binding
Alternative Name GATA4 (GATA4 ELISA Kit Abstract)
Background Synonyms: DNA binding protein GATA-GT2, GAT4, GATA binding factor-4, GATA-4, Transcription factor GATA-4
Gene Symbol: GATA4
Gene ID 2626
UniProt P43694
Research Area Hypertrophy, Lineage Markers, Transcription Factors
Pathways Peptide Hormone Metabolism, Carbohydrate Homeostasis
Plate Pre-coated
Assay Procedure
  • Remove the coating solution and wash the plate three times by filling the wells with 100 μL PBS-0.05 % Tween20. The solutions or washes are removed by flicking the plate over a sink. The remaining drops are removed by patting the plate on a paper towel.
  • Block the remaining protein-binding sites in the coated wells by adding 100 μL blocking buffer, 3 % skim milk in PBS per well. Incubate for 1 hour at RT with gentle shaking.
  • Wash the plate three times with 100 μL PBS-0.05 % Tween 20.
  • Add 50 μL of diluted antibody to each well. Incubate the plate at 37 °C for an hour with gentle shaking.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween 20.
  • Add 50 μL of conjugated secondary antibody, diluted at the optimal concentration (according to the manufacturer) in blocking buffer immediately before use. Incubate at 37 °C for an hour.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween20.
  • Prepare the substrate solution by mixing acetic acid, TMB and 0.03 % H2O2 with the volume ratio of 4:1:5.
  • Dispense 50 μL of the substrate solution per well with a multichannel pipe. Incubate the plate at 37 °C in dark for 15-30 mins.
  • After sufficient color development, add 100 μL of stop solution to the wells (if necessary).
  • Read the absorbance (optical density at 450nm) of each well with a plate reader.
Restrictions For Research Use only
Handling Advice Avoid multiple freeze-thaw cycles
Storage 4 °C
Storage Comment Store at 4 °C for frequent use, at -20°C for infrequent use.
Expiry Date 6 months
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