GATA1 ELISA Kit (GATA Binding Protein 1 (Globin Transcription Factor 1))

Details for Product GATA1 ELISA Kit No. ABIN2113844, Supplier: Log in to see
Antigen
  • ERYF1
  • GATA-1
  • GF-1
  • GF1
  • NF-E1
  • NFE1
  • XLANP
  • XLTDA
  • XLTT
  • Gata-1
  • Gf-1
  • eryf1
  • Eryf1
  • GATA1
  • gata-1
  • Gata1
  • si:dkeyp-77f7.5
  • GATA binding protein 1 (globin transcription factor 1)
  • GATA binding protein 1
  • amidase
  • aspartyl/glutamyl-tRNA amidotransferase subunit A
  • putative amidase
  • glutamyl-tRNA amidotransferase subunit A protein
  • GatA1
  • glutamyl-tRNA amidotransferase subunit A
  • hematopietic transcription factor GATA-1
  • GATA binding protein 1b
  • GATA1
  • Gata1
  • gatA1
  • gata-1
  • gata1b
  • LOC100344606
Alternatives
Human GATA1 ELISA Kit
Epitope
pSer142
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
13
11
9
2
2
2
2
1
1
1
1
1
Method Type
DNA-Binding ELISA
Application
ELISA
Options
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Analytical Method Quantitative
Detection Method Colorimetric
Specificity The GATA1 (Phospho-Ser142) DNA-Binding ELISA Kit detects endogenous levels of GATA1 only when phosphorylated at Ser142.
Characteristics Assay Type: DNA-Binding
Alternative Name GATA1 (GATA1 ELISA Kit Abstract)
Background Synonyms: Erythroid transcription factor, Eryf1, GATA-binding factor 1, GATA-1, GF-1, NF-E1 DNA-binding protein, GATA1, ERYF1, GF1
Gene Symbol: GATA1
Gene ID 2623
UniProt P15976
Research Area Organogenesis, Hematopoietic Progenitors, Transcription Factors
Pathways Cellular Response to Molecule of Bacterial Origin
Plate Pre-coated
Assay Procedure
  • Remove the coating solution and wash the plate three times by filling the wells with 100 μL PBS-0.05 % Tween20. The solutions or washes are removed by flicking the plate over a sink. The remaining drops are removed by patting the plate on a paper towel.
  • Block the remaining protein-binding sites in the coated wells by adding 100 μL blocking buffer, 3 % skim milk in PBS per well. Incubate for 1 hour at RT with gentle shaking.
  • Wash the plate three times with 100 μL PBS-0.05 % Tween 20.
  • Add 50 μL of diluted antibody to each well. Incubate the plate at 37 °C for an hour with gentle shaking.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween 20.
  • Add 50 μL of conjugated secondary antibody, diluted at the optimal concentration (according to the manufacturer) in blocking buffer immediately before use. Incubate at 37 °C for an hour.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween20.
  • Prepare the substrate solution by mixing acetic acid, TMB and 0.03 % H2O2 with the volume ratio of 4:1:5.
  • Dispense 50 μL of the substrate solution per well with a multichannel pipe. Incubate the plate at 37 °C in dark for 15-30 mins.
  • After sufficient color development, add 100 μL of stop solution to the wells (if necessary).
  • Read the absorbance (optical density at 450nm) of each well with a plate reader.
Restrictions For Research Use only
Handling Advice Avoid multiple freeze-thaw cycles
Storage 4 °C
Storage Comment Store at 4 °C for frequent use, at -20°C for infrequent use.
Expiry Date 6 months