SMAD2 ELISA Kit (SMAD, Mothers Against DPP Homolog 2)

Details for Product SMAD2 ELISA Kit No. ABIN2114377, Supplier: Log in to see
  • jv18
  • Xmad2
  • madh2
  • madr2
  • XSmad2
  • hmad-2
  • hsmad2
  • jv18-1
  • smad-2
  • fj43c06
  • wu:fj43c06
  • JV18
  • JV18-1
  • MADH2
  • MADR2
  • hMAD-2
  • hSMAD2
  • 7120426M23Rik
  • Madh2
  • Madr2
  • Smad-2
  • mMad2
  • SMAD family member 2
  • SMAD family member 2 S homeolog
  • SMAD family member 2-Z
  • SMAD2
  • smad2
  • smad2.S
  • SMAD2Z
  • Smad2
Human, Mouse (Murine), Rat (Rattus)
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Method Type
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Analytical Method Quantitative
Detection Method Colorimetric
Specificity The Smad2 (Phospho-Ser250) DNA-Binding ELISA Kit detects endogenous levels of Smad2 only when phosphorylated at Ser250.
Characteristics Assay Type: DNA-Binding
Alternative Name Smad2 (SMAD2 ELISA Kit Abstract)
Background Synonyms: JV18-1, MADH2, MADR2, Mad-related protein 2, Mothers against DPP homolog 2, Mothers against decapentaplegic homolog 2, Smad 2
Gene Symbol: SMAD2
Gene ID 4087
UniProt Q15796
Research Area Cancer, Growth Factors, Transcription Factors, Signaling
Pathways Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus
Plate Pre-coated
Assay Procedure
  • Remove the coating solution and wash the plate three times by filling the wells with 100 μL PBS-0.05 % Tween20. The solutions or washes are removed by flicking the plate over a sink. The remaining drops are removed by patting the plate on a paper towel.
  • Block the remaining protein-binding sites in the coated wells by adding 100 μL blocking buffer, 3 % skim milk in PBS per well. Incubate for 1 hour at RT with gentle shaking.
  • Wash the plate three times with 100 μL PBS-0.05 % Tween 20.
  • Add 50 μL of diluted antibody to each well. Incubate the plate at 37 °C for an hour with gentle shaking.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween 20.
  • Add 50 μL of conjugated secondary antibody, diluted at the optimal concentration (according to the manufacturer) in blocking buffer immediately before use. Incubate at 37 °C for an hour.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween20.
  • Prepare the substrate solution by mixing acetic acid, TMB and 0.03 % H2O2 with the volume ratio of 4:1:5.
  • Dispense 50 μL of the substrate solution per well with a multichannel pipe. Incubate the plate at 37 °C in dark for 15-30 mins.
  • After sufficient color development, add 100 μL of stop solution to the wells (if necessary).
  • Read the absorbance (optical density at 450nm) of each well with a plate reader.
Restrictions For Research Use only
Handling Advice Avoid multiple freeze-thaw cycles
Storage 4 °C
Storage Comment Store at 4 °C for frequent use, at -20°C for infrequent use.
Expiry Date 6 months
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