Myocyte Enhancer Factor 2A (MEF2A) ELISA Kit

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Antigen
  • MEF2A
  • xmef2a
  • ADCAD1
  • RSRFC4
  • RSRFC9
  • mef2
  • A430079H05Rik
  • adcad1
  • rsrfc4
  • rsrfc9
  • sl2
  • mef2a
  • wu:fd19d02
  • myocyte enhancer factor 2A
  • myocyte-specific enhancer factor 2A
  • myocyte-specific enhancer factor 2a
  • myocyte enhancer factor 2a
  • myocyte enhancer factor 2aa
  • MEF2A
  • EDI_092490
  • EDI_038250
  • Smp_161530
  • Smp_129430
  • mef2a
  • Mef2a
  • mef2aa
Alternatives
Human Myocyte Enhancer Factor 2A ELISA Kit
Epitope
pThr312
Reactivity
Human, Mouse (Murine), Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
12
12
12
1
1
1
Method Type
DNA-Binding ELISA
Application
ELISA
Options
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Analytical Method Quantitative
Detection Method Colorimetric
Specificity The MEF2A (Phospho-Thr312) DNA-Binding ELISA Kit detects endogenous levels of MEF2A only when phosphorylated at Thr312.
Characteristics Assay Type: DNA-Binding
Alternative Name MEF2A (MEF2A ELISA Kit Abstract)
Background Synonyms: MEF2, Myocyte-specific enhancer factor 2A, Serum response factor-like protein 1
Gene Symbol: MEF2A
Gene ID 4205
UniProt Q02078
Research Area Cardiovascular, Hypertrophy, Cardiogenesis, Transcription Factors, Neurogenesis
Pathways Neurotrophin Signaling Pathway, Activation of Innate immune Response, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades
Plate Pre-coated
Assay Procedure
  • Remove the coating solution and wash the plate three times by filling the wells with 100 μL PBS-0.05 % Tween20. The solutions or washes are removed by flicking the plate over a sink. The remaining drops are removed by patting the plate on a paper towel.
  • Block the remaining protein-binding sites in the coated wells by adding 100 μL blocking buffer, 3 % skim milk in PBS per well. Incubate for 1 hour at RT with gentle shaking.
  • Wash the plate three times with 100 μL PBS-0.05 % Tween 20.
  • Add 50 μL of diluted antibody to each well. Incubate the plate at 37 °C for an hour with gentle shaking.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween 20.
  • Add 50 μL of conjugated secondary antibody, diluted at the optimal concentration (according to the manufacturer) in blocking buffer immediately before use. Incubate at 37 °C for an hour.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween20.
  • Prepare the substrate solution by mixing acetic acid, TMB and 0.03 % H2O2 with the volume ratio of 4:1:5.
  • Dispense 50 μL of the substrate solution per well with a multichannel pipe. Incubate the plate at 37 °C in dark for 15-30 mins.
  • After sufficient color development, add 100 μL of stop solution to the wells (if necessary).
  • Read the absorbance (optical density at 450nm) of each well with a plate reader.
Restrictions For Research Use only
Handling Advice Avoid multiple freeze-thaw cycles
Storage 4 °C
Storage Comment Store at 4 °C for frequent use, at -20°C for infrequent use.
Expiry Date 6 months