MEF2D ELISA Kit (Myocyte Enhancer Factor 2D)

Details for Product MEF2D ELISA Kit No. ABIN2114548, Supplier: Log in to see
Antigen
  • sl1
  • sl-1
  • XMEF2D
  • MGC145245
  • C80750
  • myocyte enhancer factor 2D
  • MADS box transcription enhancer factor 2, polypeptide D (myocyte enhancer factor 2D)
  • mef2d
  • MEF2D
  • Mef2d
Epitope
pSer444
Reactivity
Human, Mouse (Murine), Rat (Rattus)
Method Type
DNA-Binding ELISA
Application
ELISA
Options
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Analytical Method Quantitative
Detection Method Colorimetric
Specificity The MEF2D (Phospho-Ser444) DNA-Binding ELISA Kit detects endogenous levels of MEF2D only when phosphorylated at Ser444.
Characteristics Assay Type: DNA-Binding
Alternative Name MEF2D (MEF2D ELISA Kit Abstract)
Background Synonyms: MEFD, Myocyte-specific enhancer factor 2D
Gene Symbol: MEF2D
Gene ID 4209
UniProt Q14814
Research Area Cardiovascular, Hypertrophy, Cardiogenesis, Transcription Factors, Neurogenesis
Plate Pre-coated
Assay Procedure
  • Remove the coating solution and wash the plate three times by filling the wells with 100 μL PBS-0.05 % Tween20. The solutions or washes are removed by flicking the plate over a sink. The remaining drops are removed by patting the plate on a paper towel.
  • Block the remaining protein-binding sites in the coated wells by adding 100 μL blocking buffer, 3 % skim milk in PBS per well. Incubate for 1 hour at RT with gentle shaking.
  • Wash the plate three times with 100 μL PBS-0.05 % Tween 20.
  • Add 50 μL of diluted antibody to each well. Incubate the plate at 37 °C for an hour with gentle shaking.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween 20.
  • Add 50 μL of conjugated secondary antibody, diluted at the optimal concentration (according to the manufacturer) in blocking buffer immediately before use. Incubate at 37 °C for an hour.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween20.
  • Prepare the substrate solution by mixing acetic acid, TMB and 0.03 % H2O2 with the volume ratio of 4:1:5.
  • Dispense 50 μL of the substrate solution per well with a multichannel pipe. Incubate the plate at 37 °C in dark for 15-30 mins.
  • After sufficient color development, add 100 μL of stop solution to the wells (if necessary).
  • Read the absorbance (optical density at 450nm) of each well with a plate reader.
Restrictions For Research Use only
Handling Advice Avoid multiple freeze-thaw cycles
Storage 4 °C
Storage Comment Store at 4 °C for frequent use, at -20°C for infrequent use.
Expiry Date 6 months
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