Retinoic Acid Receptor alpha ELISA Kit (Retinoic Acid Receptor, alpha)

Details for Product RARA ELISA Kit No. ABIN2114840, Supplier: Log in to see
  • rar-alpha
  • nr1b1
  • NR1B1
  • RAR
  • Nr1b1
  • RARalpha1
  • HS-RARa
  • rara2a
  • retinoic acid receptor alpha
  • retinoic acid receptor, alpha
  • retinoic acid receptor alpha L homeolog
  • LOC100136372
  • RARA
  • rara
  • Rara
  • rara.L
Human Retinoic Acid Receptor alpha ELISA Kit
Human, Mouse (Murine)
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Analytical Method Quantitative
Detection Method Colorimetric
Specificity The Retinoic Acid Receptor alpha (Phospho-Ser77) DNA-Binding ELISA Kit detects endogenous levels of Retinoic Acid Receptor alpha only when phosphorylated at Ser77.
Characteristics Assay Type: DNA-Binding
Alternative Name Retinoic Acid Receptor alpha (RARA ELISA Kit Abstract)
Background Synonyms: Retinoic acid receptor alpha, RAR-alpha, Nuclear receptor subfamily 1 group B member 1 , RARA , NR1B1
Gene Symbol: RARA
Gene ID 5914
UniProt P10276
Research Area Cancer, Transcription Factors
Pathways Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process
Plate Pre-coated
Assay Procedure
  • Remove the coating solution and wash the plate three times by filling the wells with 100 μL PBS-0.05 % Tween20. The solutions or washes are removed by flicking the plate over a sink. The remaining drops are removed by patting the plate on a paper towel.
  • Block the remaining protein-binding sites in the coated wells by adding 100 μL blocking buffer, 3 % skim milk in PBS per well. Incubate for 1 hour at RT with gentle shaking.
  • Wash the plate three times with 100 μL PBS-0.05 % Tween 20.
  • Add 50 μL of diluted antibody to each well. Incubate the plate at 37 °C for an hour with gentle shaking.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween 20.
  • Add 50 μL of conjugated secondary antibody, diluted at the optimal concentration (according to the manufacturer) in blocking buffer immediately before use. Incubate at 37 °C for an hour.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween20.
  • Prepare the substrate solution by mixing acetic acid, TMB and 0.03 % H2O2 with the volume ratio of 4:1:5.
  • Dispense 50 μL of the substrate solution per well with a multichannel pipe. Incubate the plate at 37 °C in dark for 15-30 mins.
  • After sufficient color development, add 100 μL of stop solution to the wells (if necessary).
  • Read the absorbance (optical density at 450nm) of each well with a plate reader.
Restrictions For Research Use only
Handling Advice Avoid multiple freeze-thaw cycles
Storage 4 °C
Storage Comment Store at 4 °C for frequent use, at -20°C for infrequent use.
Expiry Date 6 months
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