V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (Avian) (MAF) ELISA Kit

Details for Product No. ABIN2115041, Supplier: Log in to see
Antigen
  • MAF
  • DKFZp459D1731
  • 2810401A20Rik
  • A230108G15Rik
  • AW047063
  • c-maf
  • CCA4
  • c-MAF
  • Maf2
  • cMaf
  • Z-cmaf
  • c-Maf
  • fl13d12
  • wu:fl13d12
  • C-Maf
  • v-maf musculoaponeurotic fibrosarcoma oncogene homolog (avian)
  • avian musculoaponeurotic fibrosarcoma (v-maf) AS42 oncogene homolog
  • v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog
  • v-maf musculoaponeurotic fibrosarcoma (avian) oncogene homolog
  • MAF
  • Maf
  • maf
Reactivity
Human, Mouse (Murine), Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
10
6
2
1
1
Method Type
DNA-Binding ELISA
Application
ELISA
Options
Supplier
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Analytical Method Quantitative
Detection Method Colorimetric
Specificity The Maf DNA-Binding ELISA detects endogenous levels of total Maf protein.
Characteristics Assay Type: DNA-Binding
Alternative Name Maf (MAF ELISA Kit Abstract)
Background Synonyms: Transcription factor Maf, Proto-oncogene c-maf, C-Maf, V-maf musculoaponeurotic fibrosarcoma oncogene homolog, MAF
Gene Symbol: MAF
Gene ID 4094
UniProt O75444
Plate Pre-coated
Assay Procedure
  • Remove the coating solution and wash the plate three times by filling the wells with 100 μL PBS-0.05 % Tween20. The solutions or washes are removed by flicking the plate over a sink. The remaining drops are removed by patting the plate on a paper towel.
  • Block the remaining protein-binding sites in the coated wells by adding 100 μL blocking buffer, 3 % skim milk in PBS per well. Incubate for 1 hour at RT with gentle shaking.
  • Wash the plate three times with 100 μL PBS-0.05 % Tween 20.
  • Add 50 μL of diluted antibody to each well. Incubate the plate at 37 °C for an hour with gentle shaking.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween 20.
  • Add 50 μL of conjugated secondary antibody, diluted at the optimal concentration (according to the manufacturer) in blocking buffer immediately before use. Incubate at 37 °C for an hour.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween20.
  • Prepare the substrate solution by mixing acetic acid, TMB and 0.03 % H2O2 with the volume ratio of 4:1:5.
  • Dispense 50 μL of the substrate solution per well with a multichannel pipe. Incubate the plate at 37 °C in dark for 15-30 mins.
  • After sufficient color development, add 100 μL of stop solution to the wells (if necessary).
  • Read the absorbance (optical density at 450nm) of each well with a plate reader.
Restrictions For Research Use only
Handling Advice Avoid multiple freeze-thaw cycles
Storage 4 °C
Storage Comment Store at 4 °C for frequent use, at -20°C for infrequent use.
Expiry Date 6 months
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