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Adiponectin (ADIPOQ) ELISA Kit

Details for Product No. ABIN249421, Supplier: Log in to see
Antigen
Reactivity
Mouse (Murine), Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
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25
8
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5
3
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2
1
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Application
ELISA
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Specificity Using two antibodies this kit can assay mouse/rat high molecular weight adiponectin (HMW). Precision and reproducibility: (1) Within assay variation (2 samples, 5 replicates assay) Average C.V. is less than 5%. (2) Reproducibility (3 samples, duplicates assays, 4 days) Average C.V. is less than 5%.
Alternative Name Adiponectin HMW
Background Adiponectin, also referred to as Acrp30, AdipoQ and GBP-28, is a recently discovered 244 aminoacid protein, the product of the apM1 gene, which is sphysiologically active and specifically and highly expressed in adipose cells. The protein belongs to the soluble defence collagen superfamily, it has a collagen-like domain structurally homologous with collagen VIII and X and complement factor C1q-like globular domain. Adiponectin forms homotrimers, which are the building blocks for higher order complexes found circulating in serum. Together, these complexes make up approximately 0.01% of total serum protein. Adiponectin receptors AdipoR1 and AdipoR2 have been recently cloned, AdipoR1 is abundantly expressed in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. Paradoxically, adipose tissue-expressed adiponectin levels are inversely related to the degree of adiposity. Adiponectin concentrations correlate negatively with glucose, insulin, triglyceride concentrations, liver fat content and body mass index and positively with high-density lipoprotein-cholesterol levels, hepatic insulin sensitivity and insulin-stimulated glucose disposal. Adiponectin has been shown to increase insulin sensitivity and decrease plasma glucose by increasing tissue fat oxidation. Of particular interest is that low adiponectin serum levels predict type 2 diabetes independent of other risk factors. Adiponectin also inhibits the inflammatory processes of atherosclerosis suppressing the expression of adhesion and cytokine molecules in vascular endothelial cells and macrophages, respectively. This adipokine plays a role as a scaffold of newly formed collagen in myocardial remodelling after ischaemic injury and also stimulates angiogenesis by promoting cross-talk between AMP-activated protein kinase and Akt signalling in endothelial cells. Low serum adiponectin levels are found in patients with coronary artery disease. Moreover, high circulating levels of adiponectin are associated with decreased risk of myocardial infarction, independent of other factors. Altogether, adiponectin has the potential to become a clinically relevant parameter to be measured routinely in subjects at risk for type 2 diabetes, atherosclerosis and the metabolic syndrome.
Research Area Metabolism
Application Notes Sample requirements: 50
Restrictions For Research Use only
Buffer (A) Anti-adiponectin-coated plate - 96 wells(8x12) / 1 plate (B) Standard adiponectin solution (2000ng/ml) - 200 ul / 1 vial (C) Buffer solution - 60ml/ 1 bottle (D) HRP-conjugated anti-adiponectin - 100 ul / 1 vial (F) Chromogenic substrate reagent (TMB)
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Background publications Adachi, Brenner: "High molecular weight adiponectin inhibits proliferation of hepatic stellate cells via activation of adenosine monophosphate-activated protein kinase." in: Hepatology (Baltimore, Md.), Vol. 47, Issue 2, pp. 677-85, 2008 (PubMed).

von Eynatten, Humpert, Bluemm, Lepper, Hamann, Allolio, Nawroth, Bierhaus, Dugi: "High-molecular weight adiponectin is independently associated with the extent of coronary artery disease in men." in: Atherosclerosis, Vol. 199, Issue 1, pp. 123-8, 2008 (PubMed).

Glintborg, Frystyk, Hojlund, Andersen, Henriksen, Hermann, Hagen, Flyvbjerg, Andersen: "Total and high molecular weight (HMW) adiponectin levels and measures of glucose and lipid metabolism following pioglitazone treatment in a randomized placebo-controlled study in polycystic ovary syndrome." in: Clinical endocrinology, Vol. 68, Issue 2, pp. 165-74, 2008 (PubMed).

Nakatani, Hirose, Yamamoto, Saito, Itoh: "Significance of leptin and high-molecular weight adiponectin in the general population of Japanese male adolescents." in: Metabolism: clinical and experimental, Vol. 57, Issue 2, pp. 157-62, 2008 (PubMed).

Schraw, Wang, Halberg, Hawkins, Scherer: "Plasma adiponectin complexes have distinct biochemical characteristics." in: Endocrinology, Vol. 149, Issue 5, pp. 2270-82, 2008 (PubMed).

Sinha, Songer, Xiao, Sloan, Wang, Ji, Alborn, Davis, Swarbrick, Stanhope, Wolfe, Havel, Schraw, Konrad, Scherer, Mistry: "Analytical validation and biological evaluation of a high molecular-weight adiponectin ELISA." in: Clinical chemistry, Vol. 53, Issue 12, pp. 2144-51, 2008 (PubMed).

Westphal, Luley: "Preferential increase in high-molecular weight adiponectin after niacin." in: Atherosclerosis, Vol. 198, Issue 1, pp. 179-83, 2008 (PubMed).

Kusminski, McTernan, Schraw, Kos, OHare, Ahima, Kumar, Scherer: "Adiponectin complexes in human cerebrospinal fluid: distinct complex distribution from serum." in: Diabetologia, Vol. 50, Issue 3, pp. 634-42, 2007 (PubMed).

Andersen, Frystyk, Wolthers, Heuck, Flyvbjerg: "Gender differences of oligomers and total adiponectin during puberty: a cross-sectional study of 859 Danish school children." in: The Journal of clinical endocrinology and metabolism, Vol. 92, Issue 5, pp. 1857-62, 2007 (PubMed).

Basu, Pajvani, Rizza, Scherer: "Selective downregulation of the high molecular weight form of adiponectin in hyperinsulinemia and in type 2 diabetes: differential regulation from nondiabetic subjects." in: Diabetes, Vol. 56, Issue 8, pp. 2174-7, 2007 (PubMed).

Lo, Bernstein, Canavan, Torriani, Jackson, Ahima, Grinspoon: "Effects of TNF-alpha neutralization on adipocytokines and skeletal muscle adiposity in the metabolic syndrome." in: American journal of physiology. Endocrinology and metabolism, Vol. 293, Issue 1, pp. E102-9, 2007 (PubMed).

Beulens, van Loon, Kok, Pelsers, Bobbert, Spranger, Helander, Hendriks: "The effect of moderate alcohol consumption on adiponectin oligomers and muscle oxidative capacity: a human intervention study." in: Diabetologia, Vol. 50, Issue 7, pp. 1388-92, 2007 (PubMed).

Blueher, Brennan, Kelesidis, Kratzsch, Fasshauer, Kralisch, Williams, Mantzoros: "Total and high-molecular weight adiponectin in relation to metabolic variables at baseline and in response to an exercise treatment program: comparative evaluation of three assays." in: Diabetes Care, Vol. 30, Issue 2, pp. 280-5, 2007 (PubMed).

Asada, Yoshiji, Noguchi, Ikenaka, Kitade, Kaji, Yoshii, Yanase, Namisaki, Yamazaki, Tsujimoto, Akahane, Uemura, Fukui: "Crosstalk between high-molecular-weight adiponectin and T-cadherin during liver fibrosis development in rats." in: International journal of molecular medicine, Vol. 20, Issue 5, pp. 725-9, 2007 (PubMed).

Haqq, Muehlbauer, Svetkey, Newgard, Purnell, Grambow, Freemark: "Altered distribution of adiponectin isoforms in children with Prader-Willi syndrome (PWS): association with insulin sensitivity and circulating satiety peptide hormones." in: Clinical endocrinology, Vol. 67, Issue 6, pp. 944-51, 2007 (PubMed).

Ong, Hamilton, Sermer, Connelly, Maguire, Zinman, Hanley, Retnakaran: "Maternal serum adiponectin and infant birthweight: the role of adiponectin isoform distribution." in: Clinical endocrinology, Vol. 67, Issue 1, pp. 108-14, 2007 (PubMed).

Retnakaran, Connelly, Maguire, Sermer, Zinman, Hanley: "Decreased high-molecular-weight adiponectin in gestational diabetes: implications for the pathophysiology of Type 2 diabetes." in: Diabetic medicine : a journal of the British Diabetic Association, Vol. 24, Issue 3, pp. 245-52, 2007 (PubMed).

Modan-Moses, Stein, Pariente, Yaroslavsky, Ram, Faigin, Loewenthal, Yissachar, Hemi, Kanety: "Modulation of adiponectin and leptin during refeeding of female anorexia nervosa patients." in: The Journal of clinical endocrinology and metabolism, Vol. 92, Issue 5, pp. 1843-7, 2007 (PubMed).

Tsutamoto, Tanaka, Sakai, Ishikawa, Fujii, Yamamoto, Horie: "Total and high molecular weight adiponectin, haemodynamics, and mortality in patients with chronic heart failure." in: European heart journal, Vol. 28, Issue 14, pp. 1723-30, 2007 (PubMed).

Peake, Hughes, Shen, Charlesworth: "Glycosylation of human adiponectin affects its conformation and stability." in: Journal of molecular endocrinology, Vol. 39, Issue 1, pp. 45-52, 2007 (PubMed).