Adiponectin, High Molecular Weight (ADIPOQ) ELISA Kit

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Target Name (Antigen)
Synonyms ACDC, ACRP30, ADIPQTL1, ADPN, APM-1, APM1, GBP28, APN, Acdc, apM1, 30kDa, adipo, Acrp30, ADN
Alternatives Mouse (Murine), Rat (Rattus)
Kits with alternative reactivity to:
Pubmed 69 references available
Catalog no. ABIN249421
Quantity 96 tests
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Specificity Using two antibodies this kit can assay mouse/rat high molecular weight adiponectin (HMW). Precision and reproducibility: (1) Within assay variation (2 samples, 5 replicates assay) Average C.V. is less than 5%. (2) Reproducibility (3 samples, duplicates assays, 4 days) Average C.V. is less than 5%.
Alternative Name Adiponectin HMW
Background Adiponectin, also referred to as Acrp30, AdipoQ and GBP-28, is a recently discovered 244 aminoacid protein, the product of the apM1 gene, which is sphysiologically active and specifically and highly expressed in adipose cells. The protein belongs to the soluble defence collagen superfamily, it has a collagen-like domain structurally homologous with collagen VIII and X and complement factor C1q-like globular domain. Adiponectin forms homotrimers, which are the building blocks for higher order complexes found circulating in serum. Together, these complexes make up approximately 0.01% of total serum protein. Adiponectin receptors AdipoR1 and AdipoR2 have been recently cloned, AdipoR1 is abundantly expressed in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. Paradoxically, adipose tissue-expressed adiponectin levels are inversely related to the degree of adiposity. Adiponectin concentrations correlate negatively with glucose, insulin, triglyceride concentrations, liver fat content and body mass index and positively with high-density lipoprotein-cholesterol levels, hepatic insulin sensitivity and insulin-stimulated glucose disposal. Adiponectin has been shown to increase insulin sensitivity and decrease plasma glucose by increasing tissue fat oxidation. Of particular interest is that low adiponectin serum levels predict type 2 diabetes independent of other risk factors. Adiponectin also inhibits the inflammatory processes of atherosclerosis suppressing the expression of adhesion and cytokine molecules in vascular endothelial cells and macrophages, respectively. This adipokine plays a role as a scaffold of newly formed collagen in myocardial remodelling after ischaemic injury and also stimulates angiogenesis by promoting cross-talk between AMP-activated protein kinase and Akt signalling in endothelial cells. Low serum adiponectin levels are found in patients with coronary artery disease. Moreover, high circulating levels of adiponectin are associated with decreased risk of myocardial infarction, independent of other factors. Altogether, adiponectin has the potential to become a clinically relevant parameter to be measured routinely in subjects at risk for type 2 diabetes, atherosclerosis and the metabolic syndrome.
Research Area Metabolism
Application Notes Sample requirements: 50
Restrictions For Research Use only
Buffer (A) Anti-adiponectin-coated plate - 96 wells(8x12) / 1 plate (B) Standard adiponectin solution (2000ng/ml) - 200 ul / 1 vial (C) Buffer solution - 60ml/ 1 bottle (D) HRP-conjugated anti-adiponectin - 100 ul / 1 vial (F) Chromogenic substrate reagent (TMB)
Supplier Images
Adiponectin, High Molecular Weight (ADIPOQ) ELISA Kit Assay Procedure
Adiponectin, High Molecular Weight (ADIPOQ) ELISA Kit (2) Standard Curve
Adiponectin, High Molecular Weight (ADIPOQ) ELISA Kit (3) Cross Reactivities
General Yamauchi, Kamon, Waki et al.: "The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity." in: Nature medicine, Vol. 7, Issue 8, pp. 941-6, 2001 (PubMed).

Berg, Combs, Scherer: "ACRP30/adiponectin: an adipokine regulating glucose and lipid metabolism." in: Trends in endocrinology and metabolism: TEM, Vol. 13, Issue 2, pp. 84-9, 2002 (PubMed).

Wang, Xu, Knight et al.: "Hydroxylation and glycosylation of the four conserved lysine residues in the collagenous domain of adiponectin. Potential role in the modulation of its insulin-sensitizing activity." in: The Journal of biological chemistry, Vol. 277, Issue 22, pp. 19521-9, 2002 (PubMed).

Matsuda, Shimomura, Sata et al.: "Role of adiponectin in preventing vascular stenosis. The missing link of adipo-vascular axis." in: The Journal of biological chemistry, Vol. 277, Issue 40, pp. 37487-91, 2002 (PubMed).

Yamauchi, Kamon, Minokoshi et al.: "Adiponectin stimulates glucose utilization and fatty-acid oxidation by activating AMP-activated protein kinase." in: Nature medicine, Vol. 8, Issue 11, pp. 1288-95, 2002 (PubMed).

Okamoto, Kihara, Ouchi et al.: "Adiponectin reduces atherosclerosis in apolipoprotein E-deficient mice." in: Circulation, Vol. 106, Issue 22, pp. 2767-70, 2002 (PubMed).

Pajvani, Du, Combs et al.: "Structure-function studies of the adipocyte-secreted hormone Acrp30/adiponectin. Implications fpr metabolic regulation and bioactivity." in: The Journal of biological chemistry, Vol. 278, Issue 11, pp. 9073-85, 2003 (PubMed).

Xu, Wang, Keshaw et al.: "The fat-derived hormone adiponectin alleviates alcoholic and nonalcoholic fatty liver diseases in mice." in: The Journal of clinical investigation, Vol. 112, Issue 1, pp. 91-100, 2003 (PubMed).

Waki, Yamauchi, Kamon et al.: "Impaired multimerization of human adiponectin mutants associated with diabetes. Molecular structure and multimer formation of adiponectin." in: The Journal of biological chemistry, Vol. 278, Issue 41, pp. 40352-63, 2003 (PubMed).

Chen, Montagnani, Funahashi et al.: "Adiponectin stimulates production of nitric oxide in vascular endothelial cells." in: The Journal of biological chemistry, Vol. 278, Issue 45, pp. 45021-6, 2003 (PubMed).

Pajvani, Hawkins, Combs et al.: "Complex distribution, not absolute amount of adiponectin, correlates with thiazolidinedione-mediated improvement in insulin sensitivity." in: The Journal of biological chemistry, Vol. 279, Issue 13, pp. 12152-62, 2004 (PubMed).

Kobayashi, Ouchi, Kihara et al.: "Selective suppression of endothelial cell apoptosis by the high molecular weight form of adiponectin." in: Circulation research, Vol. 94, Issue 4, pp. e27-31, 2004 (PubMed).

Tonelli, Li, Kishore et al.: "Mechanisms of early insulin-sensitizing effects of thiazolidinediones in type 2 diabetes." in: Diabetes, Vol. 53, Issue 6, pp. 1621-9, 2004 (PubMed).

Hug, Wang, Ahmad et al.: "T-cadherin is a receptor for hexameric and high-molecular-weight forms of Acrp30/adiponectin." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 28, pp. 10308-13, 2004 (PubMed).

Waki, Yamauchi, Kamon et al.: "Generation of globular fragment of adiponectin by leukocyte elastase secreted by monocytic cell line THP-1." in: Endocrinology, Vol. 146, Issue 2, pp. 790-6, 2005 (PubMed).

Wang, Lam, Xu et al.: "Adiponectin inhibits cell proliferation by interacting with several growth factors in an oligomerization-dependent manner." in: The Journal of biological chemistry, Vol. 280, Issue 18, pp. 18341-7, 2005 (PubMed).

Xu, Chan, Hoo et al.: "Testosterone selectively reduces the high molecular weight form of adiponectin by inhibiting its secretion from adipocytes." in: The Journal of biological chemistry, Vol. 280, Issue 18, pp. 18073-80, 2005 (PubMed).

Hug, Lodish: "The role of the adipocyte hormone adiponectin in cardiovascular disease." in: Current opinion in pharmacology, Vol. 5, Issue 2, pp. 129-34, 2005 (PubMed).

Fisher, Trujillo, Hanif et al.: "Serum high molecular weight complex of adiponectin correlates better with glucose tolerance than total serum adiponectin in Indo-Asian males." in: Diabetologia, Vol. 48, Issue 6, pp. 1084-7, 2005 (PubMed).

Peake, Kriketos, Campbell et al.: "The metabolism of isoforms of human adiponectin: studies in human subjects and in experimental animals." in: European journal of endocrinology / European Federation of Endocrine Societies, Vol. 153, Issue 3, pp. 409-17, 2005 (PubMed).

Lara-Castro, Luo, Wallace et al.: "Adiponectin multimeric complexes and the metabolic syndrome trait cluster." in: Diabetes, Vol. 55, Issue 1, pp. 249-59, 2005 (PubMed).

Richards, Stephens, Charlton et al.: "Adiponectin multimerization is dependent on conserved lysines in the collagenous domain: evidence for regulation of multimerization by alterations in posttranslational modifications." in: Molecular endocrinology (Baltimore, Md.), Vol. 20, Issue 7, pp. 1673-87, 2006 (PubMed).

Nakano, Tajima, Yoshimi et al.: "A novel enzyme-linked immunosorbent assay specific for high-molecular-weight adiponectin." in: Journal of lipid research, Vol. 47, Issue 7, pp. 1572-82, 2006 (PubMed).

Wang, Lam, Chan et al.: "Post-translational modifications of the four conserved lysine residues within the collagenous domain of adiponectin are required for the formation of its high molecular weight oligomeric complex." in: The Journal of biological chemistry, Vol. 281, Issue 24, pp. 16391-400, 2006 (PubMed).

Ebinuma, Miyazaki, Yago et al.: "A novel ELISA system for selective measurement of human adiponectin multimers by using proteases." in: Clinica chimica acta; international journal of clinical chemistry, Vol. 372, Issue 1-2, pp. 47-53, 2006 (PubMed).

Richards, Hickman, Wang et al.: "Olanzapine treatment is associated with reduced high molecular weight adiponectin in serum: a potential mechanism for olanzapine-induced insulin resistance in patients with schizophrenia." in: Journal of clinical psychopharmacology, Vol. 26, Issue 3, pp. 232-7, 2006 (PubMed).

Hara, Horikoshi, Yamauchi et al.: "Measurement of the high-molecular weight form of adiponectin in plasma is useful for the prediction of insulin resistance and metabolic syndrome." in: Diabetes Care, Vol. 29, Issue 6, pp. 1357-62, 2006 (PubMed).

Bodles, Banga, Rasouli et al.: "Pioglitazone increases secretion of high-molecular-weight adiponectin from adipocytes." in: American journal of physiology. Endocrinology and metabolism, Vol. 291, Issue 5, pp. E1100-5, 2006 (PubMed).

Takemura, Osuga, Koga et al.: "Selective increase in high molecular weight adiponectin concentration in serum of women with preeclampsia." in: Journal of reproductive immunology, Vol. 73, Issue 1, pp. 60-5, 2007 (PubMed).

Chen, Chen, Hsieh et al.: "Evidence for a protective role for adiponectin in osteoarthritis." in: Biochimica et biophysica acta, Vol. 1762, Issue 8, pp. 711-8, 2006 (PubMed).

Araki, Dobashi, Kubo et al.: "High molecular weight, rather than total, adiponectin levels better reflect metabolic abnormalities associated with childhood obesity." in: The Journal of clinical endocrinology and metabolism, Vol. 91, Issue 12, pp. 5113-6, 2006 (PubMed).

Shen, Charlesworth, Kelly et al.: "Up-regulation of adiponectin, its isoforms and receptors in end-stage kidney disease." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 22, Issue 1, pp. 171-8, 2006 (PubMed).

Swarbrick, Austrheim-Smith, Stanhope et al.: "Circulating concentrations of high-molecular-weight adiponectin are increased following Roux-en-Y gastric bypass surgery." in: Diabetologia, Vol. 49, Issue 11, pp. 2552-8, 2006 (PubMed).

Salani, Briatore, Andraghetti et al.: "High-molecular weight adiponectin isoforms increase after biliopancreatic diversion in obese subjects." in: Obesity (Silver Spring, Md.), Vol. 14, Issue 9, pp. 1511-4, 2006 (PubMed).

Neumeier, Sigruener, Eggenhofer et al.: "High molecular weight adiponectin reduces apolipoprotein B and E release in human hepatocytes." in: Biochemical and biophysical research communications, Vol. 352, Issue 2, pp. 543-8, 2006 (PubMed).

Iwashima, Horio, Kumada et al.: "Adiponectin and renal function, and implication as a risk of cardiovascular disease." in: The American journal of cardiology, Vol. 98, Issue 12, pp. 1603-8, 2006 (PubMed).

Kusminski, McTernan, Schraw et al.: "Adiponectin complexes in human cerebrospinal fluid: distinct complex distribution from serum." in: Diabetologia, Vol. 50, Issue 3, pp. 634-42, 2007 (PubMed).

Blueher, Brennan, Kelesidis et al.: "Total and high-molecular weight adiponectin in relation to metabolic variables at baseline and in response to an exercise treatment program: comparative evaluation of three assays." in: Diabetes Care, Vol. 30, Issue 2, pp. 280-5, 2007 (PubMed).

Andersen, Frystyk, Wolthers et al.: "Gender differences of oligomers and total adiponectin during puberty: a cross-sectional study of 859 Danish school children." in: The Journal of clinical endocrinology and metabolism, Vol. 92, Issue 5, pp. 1857-62, 2007 (PubMed).

Suzuki, Wilson-Kubalek, Wert et al.: "The oligomeric structure of high molecular weight adiponectin." in: FEBS letters, Vol. 581, Issue 5, pp. 809-14, 2007 (PubMed).

Retnakaran, Connelly, Maguire et al.: "Decreased high-molecular-weight adiponectin in gestational diabetes: implications for the pathophysiology of Type 2 diabetes." in: Diabetic medicine : a journal of the British Diabetic Association, Vol. 24, Issue 3, pp. 245-52, 2007 (PubMed).

Semple, Halberg, Burling et al.: "Paradoxical elevation of high-molecular weight adiponectin in acquired extreme insulin resistance due to insulin receptor antibodies." in: Diabetes, Vol. 56, Issue 6, pp. 1712-7, 2007 (PubMed).

Modan-Moses, Stein, Pariente et al.: "Modulation of adiponectin and leptin during refeeding of female anorexia nervosa patients." in: The Journal of clinical endocrinology and metabolism, Vol. 92, Issue 5, pp. 1843-7, 2007 (PubMed).

Hattori, Hattori, Akimoto et al.: "Globular adiponectin activates nuclear factor-kappaB and activating protein-1 and enhances angiotensin II-induced proliferation in cardiac fibroblasts." in: Diabetes, Vol. 56, Issue 3, pp. 804-8, 2007 (PubMed).

Lo, Bernstein, Canavan et al.: "Effects of TNF-alpha neutralization on adipocytokines and skeletal muscle adiposity in the metabolic syndrome." in: American journal of physiology. Endocrinology and metabolism, Vol. 293, Issue 1, pp. E102-9, 2007 (PubMed).

Oki, Koide, Nakanishi et al.: "Fenofibrate increases high molecular weight adiponectin in subjects with hypertriglyceridemia." in: Endocrine journal, Vol. 54, Issue 3, pp. 431-5, 2007 (PubMed).

Hui, Zhang, Lee et al.: "Serum adiponectin is increased in advancing liver fibrosis and declines with reduction in fibrosis in chronic hepatitis B." in: Journal of hepatology, Vol. 47, Issue 2, pp. 191-202, 2007 (PubMed).

Ong, Hamilton, Sermer et al.: "Maternal serum adiponectin and infant birthweight: the role of adiponectin isoform distribution." in: Clinical endocrinology, Vol. 67, Issue 1, pp. 108-14, 2007 (PubMed).

Beulens, van Loon, Kok et al.: "The effect of moderate alcohol consumption on adiponectin oligomers and muscle oxidative capacity: a human intervention study." in: Diabetologia, Vol. 50, Issue 7, pp. 1388-92, 2007 (PubMed).

Tsutamoto, Tanaka, Sakai et al.: "Total and high molecular weight adiponectin, haemodynamics, and mortality in patients with chronic heart failure." in: European heart journal, Vol. 28, Issue 14, pp. 1723-30, 2007 (PubMed).

Basu, Pajvani, Rizza et al.: "Selective downregulation of the high molecular weight form of adiponectin in hyperinsulinemia and in type 2 diabetes: differential regulation from nondiabetic subjects." in: Diabetes, Vol. 56, Issue 8, pp. 2174-7, 2007 (PubMed).

Torigoe, Matsui, Ogawa et al.: "Impact of the high-molecular-weight form of adiponectin on endothelial function in healthy young men." in: Clinical endocrinology, Vol. 67, Issue 2, pp. 276-81, 2007 (PubMed).

Peake, Hughes, Shen et al.: "Glycosylation of human adiponectin affects its conformation and stability." in: Journal of molecular endocrinology, Vol. 39, Issue 1, pp. 45-52, 2007 (PubMed).

Petersen, Dufour, Savage et al.: "The role of skeletal muscle insulin resistance in the pathogenesis of the metabolic syndrome." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 31, pp. 12587-94, 2007 (PubMed).

Haqq, Muehlbauer, Svetkey et al.: "Altered distribution of adiponectin isoforms in children with Prader-Willi syndrome (PWS): association with insulin sensitivity and circulating satiety peptide hormones." in: Clinical endocrinology, Vol. 67, Issue 6, pp. 944-51, 2007 (PubMed).

Odden, Morkrid: "High molecular weight adiponectin dominates in cord blood of newborns but is unaffected by pre-eclamptic pregnancies." in: Clinical endocrinology, Vol. 67, Issue 6, pp. 891-6, 2007 (PubMed).

Schober, Neumeier, Weigert et al.: "Low molecular weight adiponectin negatively correlates with the waist circumference and monocytic IL-6 release." in: Biochemical and biophysical research communications, Vol. 361, Issue 4, pp. 968-73, 2007 (PubMed).

Liu, Retnakaran, Hanley et al.: "Total and high molecular weight but not trimeric or hexameric forms of adiponectin correlate with markers of the metabolic syndrome and liver injury in Thai subjects." in: The Journal of clinical endocrinology and metabolism, Vol. 92, Issue 11, pp. 4313-8, 2007 (PubMed).

Glintborg, Frystyk, Hojlund et al.: "Total and high molecular weight (HMW) adiponectin levels and measures of glucose and lipid metabolism following pioglitazone treatment in a randomized placebo-controlled study in polycystic ovary syndrome." in: Clinical endocrinology, Vol. 68, Issue 2, pp. 165-74, 2008 (PubMed).

Asada, Yoshiji, Noguchi et al.: "Crosstalk between high-molecular-weight adiponectin and T-cadherin during liver fibrosis development in rats." in: International journal of molecular medicine, Vol. 20, Issue 5, pp. 725-9, 2007 (PubMed).

Seino, Hirose, Saito et al.: "High molecular weight multimer form of adiponectin as a useful marker to evaluate insulin resistance and metabolic syndrome in Japanese men." in: Metabolism: clinical and experimental, Vol. 56, Issue 11, pp. 1493-9, 2007 (PubMed).

Sinha, Songer, Xiao et al.: "Analytical validation and biological evaluation of a high molecular-weight adiponectin ELISA." in: Clinical chemistry, Vol. 53, Issue 12, pp. 2144-51, 2008 (PubMed).

Nishimura, Morimoto, Matsudaira et al.: "Ratio of high-, medium-, and low-molecular weight serum adiponectin to the total adiponectin value in children." in: The Journal of pediatrics, Vol. 151, Issue 5, pp. 545-7, 547.e1-2, 2007 (PubMed).

Westphal, Luley: "Preferential increase in high-molecular weight adiponectin after niacin." in: Atherosclerosis, Vol. 198, Issue 1, pp. 179-83, 2008 (PubMed).

von Eynatten, Humpert, Bluemm et al.: "High-molecular weight adiponectin is independently associated with the extent of coronary artery disease in men." in: Atherosclerosis, Vol. 199, Issue 1, pp. 123-8, 2008 (PubMed).

Komura, Kihara, Sonoda et al.: "Clinical significance of high-molecular weight form of adiponectin in male patients with coronary artery disease." in: Circulation journal : official journal of the Japanese Circulation Society, Vol. 72, Issue 1, pp. 23-8, 2007 (PubMed).

Nakatani, Hirose, Yamamoto et al.: "Significance of leptin and high-molecular weight adiponectin in the general population of Japanese male adolescents." in: Metabolism: clinical and experimental, Vol. 57, Issue 2, pp. 157-62, 2008 (PubMed).

Schraw, Wang, Halberg et al.: "Plasma adiponectin complexes have distinct biochemical characteristics." in: Endocrinology, Vol. 149, Issue 5, pp. 2270-82, 2008 (PubMed).

Adachi, Brenner: "High molecular weight adiponectin inhibits proliferation of hepatic stellate cells via activation of adenosine monophosphate-activated protein kinase." in: Hepatology (Baltimore, Md.), Vol. 47, Issue 2, pp. 677-85, 2008 (PubMed).

Reactivities (2)
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