IL-6 ELISA Kit

Catalog No. ABIN2648701

Product Details IL-6 ELISA Kit

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivity: Mouse

Detection Method: Colorimetric

Application: ELISA

Purpose: Mouse Interleukin 6 (IL-6) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of mouse interleukin 6 (IL-6) concentrations in cell culture supernates, serum, and plasma.

Sample Type: Serum, Plasma, Cell Culture Cells

Analytical Method: Quantitative

Sensitivity: 7 pg/ml

Characteristics: Mouse Interleukin 6 (IL-6) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 3.5 h

Plate: Pre-coated

Protocol: Mouse Interleukin 6 (IL-6) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Interleukin 6 (IL-6) has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Interleukin 6 (IL-6) present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for Interleukin 6 (IL-6) is added to the wells and binds to the combination of capture antibody-IL-6 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of Interleukin 6 (IL-6) present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven Interleukin 6 (IL-6) standard dilutions and Interleukin 6 (IL-6) sample concentration determined.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL-6 (IL6 Products)

Synonyms: BSF2 ELISA Kit, HGF ELISA Kit, HSF ELISA Kit, IFNB2 ELISA Kit, IL-6 ELISA Kit, Il-6 ELISA Kit, ILg6 ELISA Kit, Ifnb2 ELISA Kit, il6 ELISA Kit, CHIL-6 ELISA Kit, interleukin 6 ELISA Kit, interleukin-6 ELISA Kit, IL6 ELISA Kit, Il6 ELISA Kit, il-6 ELISA Kit, IL-6 ELISA Kit

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome