IL12B ELISA Kit

Catalog No. ABIN2648767

Product Details IL12B ELISA Kit

Target: IL12B (Interleukin 12 beta (IL12B))

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Purpose: Human Interleukin 12p40 (IL-12p40) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interleukin 12p40 (IL-12p40) concentrations in cell culture supernates, serum, and plasma.

Sample Type: Serum, Plasma, Cell Culture Cells

Analytical Method: Quantitative

Sensitivity: 30 pg/ml

Characteristics: Human Interleukin 12p40 (IL-12p40) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 3.5 h

Plate: Pre-coated

Protocol: Human Interleukin 12p40 (IL-12p40) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-12p40 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-12p40 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-12p40 is added to the wells and binds to the combination of capture antibody- IL-12p40 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-12p40 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-12p40 standard dilutions and IL-12p40 sample concentration determined.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for IL12B

Target: IL12B (Interleukin 12 beta (IL12B))

Alternative Name: IL-12p40 (IL12B Products)

Synonyms: CLMF ELISA Kit, CLMF2 ELISA Kit, IL-12B ELISA Kit, NKSF ELISA Kit, NKSF2 ELISA Kit, p40 ELISA Kit, Il-12b ELISA Kit, Il12p40 ELISA Kit, Il-12p40 ELISA Kit, Il12 ELISA Kit, IL-12p40 ELISA Kit, IL-12 ELISA Kit, IL-12 p40 ELISA Kit, il12b ELISA Kit, il12p40.b ELISA Kit, zgc:152789 ELISA Kit, IL-12.p40 ELISA Kit, IL12p40 ELISA Kit, IL12B ELISA Kit, CLMF p40 ELISA Kit, interleukin 12B ELISA Kit, interleukin 12b ELISA Kit, interleukin 12Ba ELISA Kit, IL12B ELISA Kit, Il12b ELISA Kit, il12ba ELISA Kit

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation