GM-CSF ELISA Kit
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- Target See all GM-CSF (CSF2) ELISA Kits
- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Application
- ELISA
- Purpose
- Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) concentrations in cell culture supernates, serum, and plasma.
- Sample Type
- Serum, Plasma, Cell Culture Cells
- Analytical Method
- Quantitative
- Sensitivity
- 7 pg/ml
- Characteristics
- Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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- Discover our best selling CSF2 ELISA Kit
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- Discover our top product CSF2 ELISA Kit
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Sample Volume
- 100 μL
- Assay Time
- 3 h
- Plate
- Pre-coated
- Protocol
- This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for GM-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GM-CSF present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for GM-CSF is added to the wells and binds to the combination of capture antibody- GM-CSF in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of GM-CSF present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven GM-CSF standard dilutions and GM-CSF sample concentration determined.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
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- Target See all GM-CSF (CSF2) ELISA Kits
- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
- Alternative Name
- GM-CSF (CSF2 Products)
- Synonyms
- GMCSF ELISA Kit, GM-CSF ELISA Kit, CSF2 ELISA Kit, Csfgm ELISA Kit, Gm-CSf ELISA Kit, MGI-IGM ELISA Kit, Gm-csf ELISA Kit, Gmcsf ELISA Kit, CSF ELISA Kit, colony stimulating factor 2 ELISA Kit, colony stimulating factor 2 (granulocyte-macrophage) ELISA Kit, CSF2 ELISA Kit, Csf2 ELISA Kit
- Pathways
- JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin
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