Mouse MDSC Flow Cocktail 2

Details for Product No. ABIN2669223, Supplier: Log in to see
Reactivity
Mouse (Murine)
Clonality
Monoclonal
Application
Flow Cytometry (FACS), Isotype Control (IsoC)
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Characteristics This mouse MDSC Flow Cocktail 2 is composed of CD11b, Ly-6C, and Ly-6G antibodies. MDSCs are a heterogenous population of cells that can affect T cell function. Two different subsets of MDSC exist: CD11b+Ly-6G +Ly-6Clow (granulocytic MDSC) and CD11b+Ly-6G-Ly-6Chi (monocytic MDSC). These cells have been identified in a variety of pathological conditions including cancer and autoimmune disease. CD11b: CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen. Ly-6G: Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. Ly-6C: Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.
Components Mouse MDSC Flow Cocktail 2 with Isotype Ctrl, Rat IgG2a/Rat IgG2b/Rat IgG2c, kappa , Clone: M1/70/1A8/HK1.4 25 tests
Application Notes Optimal working dilution should be determined by the investigator.,This reagent is a multicolor cocktail of PE conjugated CD11b (clone M1/70), FITC conjugated Ly-6G (clone 1A8) and APC conjugated Ly-6C (clone HK1.4) at optimal concentration for three-color flow cytometric analysis.
Restrictions For Research Use only
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.
Supplier Images
Flow Cytometry (FACS) image for Mouse MDSC Flow Cocktail 2 (ABIN2669223) Mouse MDSC Flow Cocktail 2
Flow Cytometry (FACS) image for Mouse MDSC Flow Cocktail 2 (ABIN2669223) Mouse MDSC Flow Cocktail 2 (Image 2)
Background publications Daley, Thomay, Connolly, Reichner, Albina: "Use of Ly6G-specific monoclonal antibody to deplete neutrophils in mice." in: Journal of leukocyte biology, Vol. 83, Issue 1, pp. 64-70, 2007 (PubMed).

Dietlin, Hofman, Lund, Gilmore, Stohlman, van der Veen: "Mycobacteria-induced Gr-1+ subsets from distinct myeloid lineages have opposite effects on T cell expansion." in: Journal of leukocyte biology, Vol. 81, Issue 5, pp. 1205-12, 2007 (PubMed).

Cerwenka, Carter, Reome, Swain, Dutton: "In vivo persistence of CD8 polarized T cell subsets producing type 1 or type 2 cytokines." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 1, pp. 97-105, 1998 (PubMed).

Coxon, Rieu, Barkalow, Askari, Sharpe, von Andrian, Arnaout, Mayadas: "A novel role for the beta 2 integrin CD11b/CD18 in neutrophil apoptosis: a homeostatic mechanism in inflammation." in: Immunity, Vol. 5, Issue 6, pp. 653-66, 1997 (PubMed).

Springer: "Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm." in: Cell, Vol. 76, Issue 2, pp. 301-14, 1994 (PubMed).

Fleming, Fleming, Malek: "Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 5, pp. 2399-408, 1993 (PubMed).

Stoppacciaro, Melani, Parenza, Mastracchio, Bassi, Baroni, Parmiani, Colombo: "Regression of an established tumor genetically modified to release granulocyte colony-stimulating factor requires granulocyte-T cell cooperation and T cell-produced interferon gamma." in: The Journal of experimental medicine, Vol. 178, Issue 1, pp. 151-61, 1993 (PubMed).

Jutila, Kroese, Jutila, Stall, Fiering, Herzenberg, Berg, Butcher: "Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon-gamma." in: European journal of immunology, Vol. 18, Issue 11, pp. 1819-26, 1989 (PubMed).

Brummer, Sugar, Stevens: "Immunological activation of polymorphonuclear neutrophils for fungal killing: studies with murine cells and blastomyces dermatitidis in vitro." in: Journal of leukocyte biology, Vol. 36, Issue 4, pp. 505-20, 1984 (PubMed).